A bifunctional MXene@PtPd NPs cascade DNAzyme-mediated fluorescence/colorimetric dual-mode biosensor for Pb2+
Zhiguang Suo1, Liuyi Zhang1, Zhen Zhang1
1College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou 450001, China.
Abstract:
Pb2+ has numerous sources in cosmetics, industrial pollution and other environments. Therefore, sensitive and accurate detection of Pb2+ content is extremely important in food safety. In this work, bifunctional nanomaterials Ti3C2@PtPd NPs with fluorescence quenching effect and peroxidase activity were prepared by in situ growth of platinum‑palladium nanoparticles (PtPd NPs) on the surface of 2D material Ti3C2. Combining the DNA enzyme recognition element with magnetic separation technology, we constructed a fluorescence/colorimetric dual-channel for the sensitive detection of Pb2+. Under the optimal conditions, the detection ranges of this fluorescence/colorimetric bimodal sensing strategy were 0.1-1000 nmol/L and 0.5-1000 nmol/L, respectively. The LOD of the fluorescence method was 23 pmol/L, and that of the colorimetric method was 74 pmol/L, and the results of the detection were visible to the naked eye. This dual-mode sensing method provides a new platform for accurate, reliable and visualized detection of Pb2+.
More Related Videos
08:06The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
12:23Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
