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Updated: Jun 8, 2025
The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
A self-enhanced electrochemiluminescence aptasensor Zr-porphyrin modified with polyamidoamine for sensitive detection
Xuemei Pi1, Yuanyuan Wang2, Xianwen Kan1
1Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, Anhui Laboratory of Molecule-Based Materials, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Abstract:
Exploring novel and sensitive analysis methods for monitoring lincomycin (Lin) residues is of great significance since overuse of it would cause a serious threat to public health. Herein, a Zr-porphyrin metal-organic frameworks (Zr-TCPP) with covalently modified polyamidoamine (PAMAM) dendrimers was synthesized as a novel intramolecular self-enhanced ECL reagent, which exhibited greatly improved ECL response due to the promotion of SO4•- generation and the shortening of the electron transfer distance. Graphene oxide modified with gold nanoparticles (Au@GO) was synthesized as the quencher for the ECL sensor construction based on the quenching strategy. The present aptasensor achieved a wide linear range of 1.0 × 10-14 - 5.0 × 10-9 g/mL and a low detection limit of 1.7 fg/mL, which was applied for the determination of Lin in different real samples with satisfactory results.
