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Author Correction: Large-scale benchmarking of circRNA detection tools reveals large differences in sensitivity but not in precision.

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Identification of Circular RNAs using RNA Sequencing
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Current Understandings and Open Hypotheses on Extracellular Circular RNAs.

Jasper Verwilt1,2, Marieke Vromman3

  • 1Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium.

Wiley Interdisciplinary Reviews. RNA
|November 7, 2024
PubMed
Summary

Extracellular circular RNAs (excircRNAs) show promise as disease biomarkers due to their stability in biofluids. However, research faces challenges in nomenclature and standardized protocols for excircRNA analysis.

Keywords:
circRNAcircular RNAextracellular spaceextracellular vesicles

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genomics

Background:

  • Circular RNAs (circRNAs) are stable RNA molecules implicated in various diseases, including cancer.
  • Cells release circRNAs into extracellular environments, forming extracellular circRNAs (excircRNAs).
  • ExcircRNAs are found in biofluids like blood and urine, offering potential as disease biomarkers.

Purpose of the Study:

  • To summarize current knowledge on extracellular circRNAs (excircRNAs).
  • To identify and discuss challenges in the field of excircRNA research.
  • To outline future directions for improving excircRNA studies.

Main Methods:

  • Literature review and synthesis of existing research on circRNAs and excircRNAs.
  • Analysis of challenges related to nomenclature and experimental protocols.
  • Identification of research gaps and future research needs.

Main Results:

  • ExcircRNAs are stable and abundant in biofluids, suggesting significant biomarker potential.
  • The field of excircRNA research is hindered by complex biological systems, inconsistent nomenclature, and varied protocols.
  • Standardization of methods and clear nomenclature are crucial for advancing excircRNA research.

Conclusions:

  • Extracellular circRNAs (excircRNAs) represent a promising area for biomarker discovery.
  • Overcoming current challenges in nomenclature and methodology is essential for realizing the potential of excircRNAs.
  • Further research is needed to standardize protocols and validate excircRNAs as reliable biomarkers.