Related Experiment Video
Updated: Jun 8, 2025
Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements
Published on: May 13, 2014
"Ayoub-Shklar" as a Special Stain for Keratin: A Histopathological Study
Raman Dineja1, Harikrishnan Prasad2, KenniyanKumar Sri Chinthu2
1Department of Oral Pathology and Oral Microbiology, SRM Dental College, Chennai, Tamil Nadu, India.
Background:
Special stains in histopathological studies are used to identify the structures with different dyes apart from the routine stain hematoxylin and Eosin (H and E). The component which is present in the special stains will have a specific bond and affinity for particular tissue components in the histological specimen. Structures like keratin takes up an eosinophilic stain in routine (H and E) staining. Most of the potentially malignant disorders and carcinomas arise due to the keratinization defect, which makes keratin an important diagnostic tool. There are different stains such as Ayoub-Shklar (A-S), Dane-Herman (D-H), and rapid Papanicolaou (PAP) which is used to identify keratin. In A-S stain, keratins can be stained in magenta-red and orange colors.
Aim:
we compared A-S special stain and routine stain in terms of staining intensity or quality, the pattern of staining, and specificity for staining keratin.
Materials And Methods:
Thirty cases from the department archives that included 10 well-differentiated squamous cell carcinoma, 10 verrucous carcinoma, and 10 epithelial dysplasia were taken and each case was stained with both A-S and H and E stain.
Results:
A-S showed an almost equal distribution of uniform and patchy staining patterns, but H and E showed more patchy staining patterns in the three groups. H and E stain shows good staining quality than A-S. A-S shows almost 90% of satisfactory staining specificity.
Conclusion:
Special stain like A-S stain can be used to stain keratin in different color, but H and E always remain gold standard stain.
More Related Videos
12:01Techniques for Processing Eyes Implanted With a Retinal Prosthesis for Localized Histopathological Analysis
Published on: August 2, 2013
07:36Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice
Published on: September 26, 2018
Related Concept Videos
01:13Special Staining Techniques
01:03Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
01:26Differential Staining Technique
01:24Simple Staining Technique