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The inhibition effect of psoralen on prostate cancer PC3 cells via down-regulation of long non-coding RNA

Shushang Chen1,2, Mingfang Weng2, Ronghui Lin1

  • 1Department of Urology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, China.

Translational Andrology and Urology
|November 7, 2024
PubMed
Abstract

Insights

Psoralen inhibits castration-resistant prostate cancer (CRPC) cell growth by arresting the cell cycle and down-regulating lncRNA ENST00000510619. This suggests psoralen

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Castration-resistant prostate cancer (CRPC) requires novel therapeutic agents.
  • Psoralen exhibits anti-cancer properties, but its role in prostate cancer (PCa) is underexplored.
  • This study investigates psoralen's effects on PC3 prostate cancer cells.

Purpose of the Study:

  • To evaluate the anti-cancer effects of psoralen on PC3 cells.
  • To elucidate the underlying molecular mechanisms, including gene expression changes and long non-coding RNA (lncRNA) involvement.
  • To assess the potential of psoralen and lncRNA ENST00000510619 as therapeutic targets for CRPC.

Main Methods:

  • Cell proliferation assessed by Cell Counting Kit-8 (CCK-8) assay.
  • Cell cycle progression analyzed via flow cytometry.
  • Differential gene expression profiles determined using microarray analysis.
  • lncRNA ENST00000510619 expression quantified by RT-qPCR.
  • Cell viability, invasion, and migration evaluated after psoralen or si-lncRNA ENST00000510619 treatment.

Main Results:

  • Psoralen significantly inhibited PC3 cell proliferation in a dose- and time-dependent manner.
  • Psoralen induced G1 and G2/M phase cell cycle arrest.
  • Microarray analysis revealed significant differential expression of numerous lncRNAs and mRNAs.
  • Psoralen down-regulated lncRNA ENST00000510619 expression.
  • Both psoralen and si-lncRNA ENST00000510619 inhibited PC3 cell viability, invasion, and migration.

Conclusions:

  • Psoralen effectively inhibits PC3 cell proliferation and induces cell cycle arrest in vitro.
  • The anti-cancer mechanism involves modulation of multiple lncRNAs and mRNAs, notably the down-regulation of lncRNA ENST00000510619.
  • Psoralen shows promise as a novel anti-CRPC therapeutic, with lncRNA ENST00000510619 as a potential clinical target.
Keywords:
PsoralenlncRNA ENST00000510619long non-coding RNA (lncRNA)microarray analysisprostate cancer (PCa)

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