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Updated: Jun 8, 2025
Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
Published on: October 20, 2021
A Sensitive and Quick Fluorescent Sensor for the "Turn-On" Detection and Imaging of Glutathione Based on Sulfur
Zhenlin Hu1, Min Cheng1, Yuanyan Zhang1
1College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Abstract:
Glutathione (GSH) is one of the most abundant bioethanol antioxidants in living cells. Here, a fluorescent probe based on MnO2 nanosheets and sulfur quantum dots (SQDs) was fabricated. Because of the synergistic effect of IFE and FRET, the fluorescence from SQDs was quenched by MnO2 nanosheets. In the presence of GSH, the fluorescence of SQDs could be recovered because of the reduction of MnO2 nanosheets by GSH. The method can detect GSH in the concentration range of 5 ~ 1000 μM with the detection limit as low as 1.26 μM. This quick, easy, and cost-effective sensor could be used for the quantification of GSH in serum samples and the imaging of GSH in Escherichia coli O157:H7.
