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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Related Experiment Video

Updated: Jun 8, 2025

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Published on: August 2, 2018

Engineered Cardiac Tissues as a Platform for CRISPR-Based Mitogen Discovery.

Sophia DeLuca^1,2, Nicholas Strash^1,2, Yifan Chen¹

¹Department of Biomedical Engineering, Duke University, Durham, NC, 27708, USA.

Advanced Healthcare Materials

|November 7, 2024

Summary

This summary is machine-generated.

Researchers identified a new way to stimulate cardiomyocyte proliferation by targeting adenosine deaminase. This discovery could advance heart regeneration and improve stem cell-derived cardiomyocyte maturation.

Keywords:

CRISPR cardiomyocyte maturation proliferation tissue engineering

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Last Updated: Jun 8, 2025

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Published on: August 2, 2018

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

Published on: March 1, 2016

Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing

Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing

Published on: March 19, 2013

Area of Science:

Cardiovascular Biology
Regenerative Medicine
Molecular Cardiology

Background:

Understanding cardiomyocyte (CM) cell cycle regulation is crucial for cardiac repair and maturation of stem cell-derived CMs.
Gene therapies offer potential for controlled CM proliferation, but identifying effective mitogens requires robust in vitro methods.

Purpose of the Study:

To develop and apply a genetic and tissue engineering platform for discovering and validating novel cardiac mitogens.
To identify specific genetic modifications that promote cardiomyocyte proliferation and maturation in vitro.

Main Methods:

Established a CRISPR knockout screening strategy in neonatal rat ventricular myocyte (NRVM) monolayers.
Validated candidate mitogens using 3-D engineered cardiac tissues (ECTs).
Utilized RNA-sequencing to identify molecular pathways involved in CM cycling.

Main Results:

Identified knockout of adenosine deaminase (ADA-KO) as a potent pro-mitogenic stimulus for CMs.
Discovered increased pentose phosphate pathway (PPP) activity as the key mechanism driving ADA-KO-induced CM cycling.
Demonstrated that modulating glucose-6-phosphate dehydrogenase (G6PD) activity directly impacts CM proliferation.

Conclusions:

The developed platform enables efficient in vitro discovery and validation of cardiomyocyte mitogens.
Targeting purine metabolism and the pentose phosphate pathway presents a promising strategy for promoting cardiac regeneration and maturation.
This research accelerates translational efforts in cardiac regenerative medicine.