Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

623
Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
623
Coagulation01:09

Coagulation

4.9K
The coagulation phase is a critical part of the body's process to prevent blood loss following injury to blood vessels. It involves chemical reactions that form a clot to seal the injured area. The clotting process begins shortly after injury, within 15-20 seconds for severe damage and 1-2 minutes for minor injuries.
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...
4.9K
Introduction to Hemostasis01:05

Introduction to Hemostasis

5.9K
Hemostasis is a complex physiological process that prevents excessive bleeding when a blood vessel is injured. It's crucial for maintaining the integrity of the circulatory system, as it ensures that our blood remains fluid while still within the vascular network and yet clots to prevent blood loss upon vessel injury.
The three phases of hemostasis involve many clotting factors present in plasma and several substances released by platelets and injured tissue cells. It is a fast, localized,...
5.9K
Clot Retraction and Fibrinolysis01:16

Clot Retraction and Fibrinolysis

4.2K
After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
4.2K
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

4.6K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
4.6K
Extrinsic and Intrinsic Pathways of Hemostasis01:20

Extrinsic and Intrinsic Pathways of Hemostasis

5.7K
Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photolabile-Protected Triazine-Based Janus G-C and Triple G-C-T Base-Coded Nucleobases for Supramolecular Polymer Construction.

The Journal of organic chemistry·2026
Same author

Hierarchically Porous Multivariate COF Aerogel With Enhanced Diffusibility and Tailored Functionality for Highly Efficient Bromine Sequestration.

Angewandte Chemie (International ed. in English)·2026
Same author

Impact of design optimization on cyclic loading performance of novel pedicle screw and comparison with control device: a bilayer model simulation study.

Medical engineering & physics·2026
Same author

Fast self-healing in a layered molecular crystal mediated by stress-induced symmetry breaking.

Nature communications·2026
Same author

Influence of hydrophilic/hydrophobic diols on the properties of polyurethane hydrogels: solvent-free one-pot synthesis.

Journal of materials chemistry. B·2025
Same author

Critical advances in biofabrication and biomaterial strategies in tracheal tissue engineering: A comprehensive overview.

Advances in colloid and interface science·2025

Related Experiment Video

Updated: Jun 8, 2025

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli
13:10

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli

Published on: September 25, 2016

9.9K

Thrombin Immobilized Hemocompatible Radiopaque Polyurethane Microspheres for Topical Blood Coagulation.

Sonali S Naik1,2, Arun Torris1, Gorakh Hiraman Ghuge1,2

  • 1Polymer Science and Engineering Division, CSIR-National Chemical Laboratory, Pune, India.

Journal of Biomedical Materials Research. Part A
|November 7, 2024
PubMed
Summary

New radiopaque microspheres improve embolization therapy by enabling noninvasive monitoring and enhancing hemostasis. Thrombin immobilization provides unique radiopacity and hemostatic properties for better embolization efficiency.

Keywords:
blood coagulationmicrospheresmicro‐CTpolyurethaneradiopaquethrombin

More Related Videos

Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging
10:50

Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging

Published on: June 29, 2013

12.3K
A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

13.7K

Related Experiment Videos

Last Updated: Jun 8, 2025

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli
13:10

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli

Published on: September 25, 2016

9.9K
Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging
10:50

Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging

Published on: June 29, 2013

12.3K
A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

13.7K

Area of Science:

  • Biomaterials Science
  • Interventional Radiology
  • Polymer Chemistry

Background:

  • Microspheres are increasingly used for embolization, but challenges like noninvasive monitoring and reflux-induced complications persist.
  • Current embolic agents lack inherent radiopacity, hindering real-time tracking during procedures.
  • Unintentional occlusions due to reflux limit the safety and efficacy of embolization therapy.

Purpose of the Study:

  • To develop novel radiopaque microspheres for improved embolization procedures.
  • To address limitations of current embolic agents, including poor traceability and risk of reflux.
  • To create microspheres with enhanced hemostatic properties and noninvasive monitoring capabilities.

Main Methods:

  • Synthesis of an iodinated monomer (IBHV) for radiopacity and functionality.
  • Fabrication of radiopaque polyurethane microspheres (average diameter 474 ± 73 μm).
  • Immobilization of thrombin onto the microsphere surface to enhance hemostatic effects.

Main Results:

  • Successfully synthesized IBHV and fabricated smooth-surfaced, radiopaque microspheres.
  • Microspheres demonstrated non-cytotoxicity and acceptable hemolysis rates.
  • Enhanced traceability via X-ray imaging and improved hemostatic effect after thrombin immobilization.

Conclusions:

  • Developed specialty microspheres with intrinsic radiopacity and surface functionality for embolization.
  • Thrombin-immobilized microspheres offer enhanced hemostasis and traceability, improving embolization efficiency.
  • These advancements pave the way for safer and more effective embolization therapies.