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Rapid, Affordable, and Uncomplicated Production of Bacterial Cell-free Lysate
Published on: October 29, 2021
Regulatory Components for Bacterial Cell-Free Systems Engineering
Pao-Wan Lee1, Sebastian J Maerkl1
1Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne, Lausanne 1015, Switzerland.
Abstract:
Cell-free systems are advancing synthetic biology through fast prototyping and modularity. Complex regulatory networks can now be implemented in cell-free systems enabling various applications, such as diagnostic tool development, gene circuit prototyping, and metabolic engineering. As functional complexity increases, the need for regulatory components also grows. This review provides a comprehensive overview of native as well as engineered regulatory components and their use in bacterial cell-free systems.
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