Mechanism of antimony oxidation and adsorption using immobilized Klebsiella aerogenes HC10 in soil

Klebsiella aerogenes HC10 is one of the few strains isolated from contaminated soil that efficiently oxidizes Sb. However, the sensitivity of microorganisms to environmental conditions limits Sb-oxidizing bacteria applications in soil remediation. Immobilizing Sb-oxidizing bacteria is a promising strategy to improve colonization rates and microorganism inefficiencies and to strengthen bioremediation in Sb-contaminated soil. This study evaluated the feasibility of an immobilization approach to enhance Sb oxidation and the remediation performance of strain HC10 in soil. The results indicated that a mixed matrix of polyvinyl alcohol and sodium alginate as fixed carriers provided a porous microstructural environment conducive to HC10 colonization and proliferation. Sb(III) concentration was reduced by 9.8 mg/L. The total Sb decreased by 3.8 mg/L by immobilized HC10 after 7 d. Key metabolites involved in Sb oxidation and adsorption were significantly upregulated. In soil, immobilized HC10 removed 48.68 % and 61.74 % of water-extractable and citric acid-extractable Sb(III), respectively. Some well-crystallized (hydr)oxide Sb fractions binding to the mineral surface were transformed into the mineral lattice form, creating an inner-sphere complex that effectively immobilized Sb. Immobilized HC10 enhanced hydrogen peroxidase, urease, and sucrase activities related to soil antioxidants and nutrient cycling. Immobilized HC10 promoted the proliferation of indigenous bacteria, which emerged as the dominant bacterial community with the potential for Sb oxidation. The ars operon genes associated with Sb resistance and transport were significantly expressed in HC10 treatments, providing a crucial basis for colonization in the soil. These results highlight the potential of immobilized Sb-oxidizing bacteria for enhanced bioremediation of Sb-contaminated soil.