Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.8K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.8K
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

1.4K
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Intravenous thrombolysis for acute central retinal artery occlusion: Protocol for a systematic review and individual participant data meta-analysis of randomized controlled trials.

PloS one·2026
Same author

Multimodal Molecular Mapping of the Vasculature in Human Cortex Reveals Lipid Markers of Cerebral Amyloid Angiopathy.

bioRxiv : the preprint server for biology·2026
Same author

Intravenous thrombolysis for acute central retinal artery occlusion: Protocol for a systematic review and individual participant data meta-analysis of randomized controlled trials.

PloS one·2026
Same author

A Multimodal Imaging Pipeline for the Discovery of Molecular Markers of Cellular Neighborhoods.

bioRxiv : the preprint server for biology·2026
Same author

Triptan Initiation and Cerebrovascular Events in Patients With Migraine: A Nationwide Cohort Study.

Journal of the American Heart Association·2026
Same author

Signal Strength Aware Latent Spaces Reveal Molecularly Distinct Substructures within Human Kidney Tissue.

bioRxiv : the preprint server for biology·2026
Same journal

How "Soft" Are Your Gas Mixtures? Effects of Modifier Gas Types on the Dissociation of Labile Ions in Differential Mobility Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same journal

A Robotic Sample Handling Platform for Fully Automated Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Direct Analysis in Real-Time Tandem Mass Spectrometry for Rapid Screening of Thirty-one Plant Growth Regulator Residues in <i>Rehmannia glutinosa</i>.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Characterization of Alkane Oxidation Products in a Corona-Discharge Reactor Using Ammonia-Doped Ion Mobility-Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Integration of a Modified Synchrotron Radiation Photoionization Time-of-Flight Mass Spectrometer with a Residual Gas Analyzer for Complementary Detection of Catalytic Products with Different Ionization Energies.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Screen for Tissue-Specific Markers of Drug-Induced Phospholipidosis Using Mass Spectrometry Imaging.

Journal of the American Society for Mass Spectrometry·2026
See all related articles

Related Experiment Video

Updated: May 6, 2026

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

14.2K

A Streamlined Workflow for Microscopy-Driven MALDI Imaging Mass Spectrometry Data Collection.

Allison B Esselman1,2, Megan S Ward1,3, Cody R Marshall1,3

  • 1Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee 37240, United States.

Journal of the American Society for Mass Spectrometry
|November 7, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a workflow for precise tissue sampling in matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS). It optimizes data acquisition by targeting specific regions of interest (ROIs), enhancing efficiency and throughput in biomedical research.

Keywords:
HeLa cellshigh spatial resolution imaginghigh-throughputhuman brainhuman kidneymolecular imagingmultimodalsingle-cell analysistargetedtargeted sampling

More Related Videos

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
09:49

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Published on: November 18, 2022

2.0K
Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells
08:48

Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells

Published on: May 23, 2025

107

Related Experiment Videos

Last Updated: May 6, 2026

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

14.2K
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
09:49

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Published on: November 18, 2022

2.0K
Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells
08:48

Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells

Published on: May 23, 2025

107

Area of Science:

  • Biomedical research
  • Molecular imaging
  • Mass spectrometry

Background:

  • Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) is crucial for biomedical research.
  • Increasing spatial resolution in MALDI IMS leads to longer acquisition times, larger file sizes, and higher costs.
  • Precise sampling of targeted tissue regions is essential to optimize information gain and minimize resource expenditure.

Purpose of the Study:

  • To develop and demonstrate a workflow for precise, microscopy-guided region selection in MALDI IMS.
  • To enable automated sampling of specific regions of interest (ROIs) based on tissue features.
  • To enhance efficiency and throughput in molecular imaging studies.

Main Methods:

  • A workflow integrating standard software for direct sampling of microscopy-defined regions by MALDI IMS was developed.
  • Case studies included manual annotation of vasculature in human brain tissue.
  • Automated segmentation algorithms were used for renal tissue units and dispersed HeLa cells.

Main Results:

  • The workflow successfully minimized data acquisition from unnecessary sample regions.
  • Significant increases in experimental throughput were achieved.
  • Molecular heterogeneity within targeted ROIs was effectively uncovered.

Conclusions:

  • This approach provides an accessible method for spatially targeted MALDI IMS.
  • It facilitates multimodal molecular imaging studies by integrating microscopy and MALDI IMS.
  • The workflow optimizes resource utilization and enhances biological information retrieval from MALDI IMS experiments.