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Related Experiment Videos

A better method for terminal bleeding of mice.

A J Adeghe, J Cohen

    Laboratory Animals
    |January 1, 1986
    PubMed
    Summary

    A novel, reliable method for exsanguinating mice under carbon dioxide (CO2) anesthesia yields large volumes of high-quality blood. This technique ensures minimal hemolysis and contamination, offering a quick and efficient procedure for researchers.

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    Area of Science:

    • Veterinary Medicine
    • Animal Research Methodology
    • Laboratory Animal Science

    Background:

    • Efficient and ethical animal research requires reliable methods for biological sample collection.
    • Traditional exsanguination techniques in mice can lead to sample contamination or hemolysis, compromising data integrity.
    • There is a need for improved protocols that maximize blood yield while minimizing animal distress.

    Purpose of the Study:

    • To describe a new, reliable, and efficient method for exsanguinating mice.
    • To evaluate the quality (hemolysis, contamination) and quantity of blood obtained using the novel technique.
    • To establish a reproducible protocol for researchers requiring large volumes of mouse blood.

    Main Methods:

    • The described procedure involves exsanguination of mice under carbon dioxide (CO2) anesthesia.
    • Venepuncture of the caudal (inferior) vena cava is performed.
    • Gentle aspiration of blood into a syringe is utilized to collect samples.

    Main Results:

    • The novel technique consistently yields large volumes of blood, ranging from 1.60 to 2.50 ml per mouse.
    • Blood samples collected were free from hemolysis.
    • Samples were also free from contamination, indicating a high-quality yield.

    Conclusions:

    • The described method provides an easy, quick, and reliable technique for mouse exsanguination.
    • This method ensures a high yield of pure, uncontaminated blood, suitable for various research applications.
    • The protocol offers a significant improvement over existing methods for collecting mouse blood samples.

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