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Related Experiment Video

Updated: Jun 8, 2025

Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

Published on: October 16, 2013

Leukocyte rolling on engineered nanodot surfaces.

X Lin-Schmidt¹, A S W Ham¹, M L Reed²

¹Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22908, USA.

Micro & Nano Letters

|November 8, 2024

Summary

This summary is machine-generated.

Nanofabricated substrates with patterned P-selectin revealed that leukocyte rolling velocity and pausing behavior depend on nanodot spacing and wall shear stress, offering insights into inflammation.

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Last Updated: Jun 8, 2025

Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

Published on: October 16, 2013

Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns

Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns

Published on: February 13, 2011

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Published on: September 27, 2021

Area of Science:

Biophysics
Cellular Biology
Biomaterials

Background:

Leukocyte rolling initiates inflammation by adhering to endothelial cells.
Understanding leukocyte-endothelial interactions is crucial for inflammation research.

Purpose of the Study:

To investigate how nanometer-scale adhesion patterns influence leukocyte rolling dynamics.
To analyze the effect of P-selectin distribution and wall shear stress on cell adhesion.

Main Methods:

Fabrication of nanopatterned substrates with varying feature sizes and spacings.
Immobilization of P-selectin onto nanopatterned dots.
Analysis of HL60 cell rolling behavior under different wall shear stresses.

Main Results:

Leukocyte rolling velocity increased with nanodot spacing at shear stresses > 2 dyne/cm².
Pattern spacing influenced rolling pauses (waiting time) and velocity variations at higher shear stresses.
Nanopatterning affected both average rolling velocity and the saltatory nature of cell adhesion.

Conclusions:

Nanofabricated substrates provide a tool to study the biophysical mechanisms of leukocyte adhesion.
Spatial arrangement of adhesion molecules significantly impacts dynamic leukocyte-endothelial interactions.
These findings contribute to understanding the early stages of inflammatory processes.