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Related Experiment Video

Updated: Jun 8, 2025

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

Fish Cell Spheroids, a Promising In Vitro Model to Mimic In Vivo Research: A Review.

Antonio Gómez-Mercader¹, Luis Monzón-Atienza¹, Daniel Montero¹

¹Grupo de Investigación en Acuicultura (GIA), Instituto Universitario en Acuicultura Sostenible y Ecosistemas Marinos (IU-ECOAQUA), Universidad de Las Palmas de Gran Canaria (ULPGC), 35214 Telde, Spain.

|November 8, 2024

Summary

This summary is machine-generated.

Three-dimensional (3D) fish cell cultures, specifically spheroids, offer a promising in vitro model to mimic in vivo conditions. This review highlights methods for generating these spheroids and their diverse applications in toxicology, drug screening, and more.

Keywords:

3D culture bioethics fish cell lines fish model in vitro culture spheroids

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A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines

A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines

Published on: January 20, 2023

Area of Science:

Cell Biology
Aquatic Toxicology
Biotechnology

Background:

In vitro cell culture is crucial for studying biological processes and cellular mechanisms.
Three-dimensional (3D) cell cultures, particularly spheroids, provide a more physiologically relevant alternative to traditional 2D monolayer cultures.
The use of 3D fish cell cultures is expanding beyond basic characterization to explore complex biological questions.

Purpose of the Study:

To review the primary methods used for generating fish cell spheroids.
To summarize the current applications of fish cell spheroids in various research fields.
To highlight the potential of fish cell spheroids for future scientific advancements.

Main Methods:

Spheroid generation techniques for fish cells.
Methods for culturing and maintaining 3D fish cell aggregates.
Analysis of spheroid morphology and physiology.

Main Results:

Spheroids effectively mimic in vivo conditions for fish cells.
Established methods allow for the generation of various fish cell spheroids.
Diverse applications are emerging, including eco-toxicology, drug screening, and endocrine studies.

Conclusions:

Fish cell spheroids represent a powerful tool for in vitro research, bridging the gap between 2D cultures and live animal studies.
Further development and application of these 3D models will significantly advance aquatic research.
Spheroid technology holds great promise for understanding fish biology and improving environmental and pharmaceutical testing.