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Lamination-based organoid spatially resolved transcriptomics technique for primary lung and liver organoid
Shaohua Ma1, Wanlong Wang1, Jiaqi Zhou1
1Tsinghua Shenzhen International Graduate School and Tsinghua-Berkeley Shenzhen Institute, Tsinghua University, Shenzhen 518055, China.
Summary
A new lamination-based organoid spatially resolved transcriptomics (LOSRT) method enables detailed cell mapping in primary organoids. This technique preserves tissue structure, allowing for precise characterization of lung and liver organoids.
Area of Science:
- Biotechnology
- Genomics
- Organoid Research
Background:
- Spatial transcriptomics excels in characterizing complex tissues and organoids.
- Standard sectioning methods are unsuitable for primary tissue-derived organoids, limiting their spatial transcriptomic analysis.
- A gap exists in applying spatial transcriptomics to primary organoids.
Purpose of the Study:
- To develop and validate a novel spatial transcriptomics technique for primary tissue-derived organoids.
- To demonstrate the efficacy of lamination-based organoid spatially resolved transcriptomics (LOSRT) in preserving organoid integrity and cellular structures.
- To enable detailed cellular mapping and characterization of primary organoids.
Main Methods:
- Organoid formulation using droplet-engineering.
- Lamination of primary mouse lung and liver organoids using a weight-compression device.
- Application of lamination-based organoid spatially resolved transcriptomics (LOSRT) for high-resolution spatial gene expression profiling.
- Validation using immunohistology to confirm cell distribution and counts.
Main Results:
- LOSRT successfully preserved cellular integrity and delicate epithelium structures within laminated organoids.
- Detailed spatial transcriptomic profiles of mouse lung and liver organoids were generated, identifying diverse cell types.
- Cellular composition, including alveolar cells, hepatocytes, and macrophages, was accurately mapped.
- Immunohistology confirmed the spatial distribution and counts of identified cell populations.
Conclusions:
- LOSRT provides an automated and integrated method for spatial transcriptomics in primary organoids.
- This technique overcomes limitations of traditional methods for organoid analysis.
- LOSRT has the potential to standardize and accelerate the characterization of primary tissue-derived organoids for research and therapeutic applications.
