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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Jun 8, 2025

Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans

Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans

Published on: August 6, 2019

Protocol for isolating specific C. elegans neuron types for bulk and single-cell RNA sequencing.

Seth R Taylor¹, Rebecca D McWhirter¹, Brittany K Matlock²

¹Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37240, USA.

|November 8, 2024

Summary

This summary is machine-generated.

This study details a protocol for isolating specific neuron types in C. elegans for gene expression analysis. The method uses fluorescence-activated cell sorting (FACS) for precise cell isolation in both larval and adult worms.

Keywords:

cell biology genomics model organisms neuroscience

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Last Updated: Jun 8, 2025

Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans

Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans

Published on: August 6, 2019

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Published on: October 12, 2018

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

Area of Science:

Neuroscience
Developmental Biology
Genomics

Background:

The Caenorhabditis elegans nervous system's compact and well-characterized nature makes it an excellent model for studying neural development.
Understanding neuron-specific gene expression is crucial for deciphering neural development, function, and connectivity.

Purpose of the Study:

To present a detailed protocol for isolating specific neuron types from C. elegans.
To enable gene expression profiling through bulk or single-cell RNA sequencing of isolated neurons.

Main Methods:

Worm synchronization and dissociation techniques.
Fluorescence-activated cell sorting (FACS) for precise isolation of specific neuron types.
RNA extraction and preparation for both bulk and single-cell RNA sequencing.

Main Results:

A robust protocol for isolating individual neuron types from C. elegans.
The protocol is effective for both larval and adult developmental stages.
The isolated cells are suitable for downstream gene expression analysis.

Conclusions:

This protocol provides a valuable tool for researchers studying C. elegans neurobiology.
It facilitates in-depth analysis of neuron-specific gene expression patterns.
Enables a deeper understanding of genetic programs governing neuronal development and function.