Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

49.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
49.5K
CRISPR and crRNAs02:53

CRISPR and crRNAs

16.8K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.8K
What is Genetic Engineering?00:49

What is Genetic Engineering?

73.7K
Overview
73.7K
Homologous Recombination02:31

Homologous Recombination

50.2K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA signatures preserved in the official 1978 sample collection of the Shroud of Turin.

Scientific reports·2026
Same author

Genotyping of Commercial European <i>Cannabis</i> Seeds Based on Multiple Mapped Marker Loci: A Comparative Study of Drug and Hemp Varieties.

Plants (Basel, Switzerland)·2025
Same author

PME10 Is a Pectin Methylesterase Driving PME Activity and Immunity Against Botrytis cinerea in Grapevine (Vitis vinifera L.).

Plant biotechnology journal·2025
Same author

Female gametophyte development, pollen‒pistil interactions and embryogenic patterns in chicory (Cichorium intybus): a self-incompatibility perspective.

Plant cell reports·2025
Same author

Reappraisal of the Genetic Diversity Patterns in <i>Puya raimondii</i>-The Queen of the Andes: Insights from Molecular Marker Analysis Reveal an Inbreeding Reproductive Strategy.

Plants (Basel, Switzerland)·2025
Same author

The Transcriptional Landscape of Berry Skin in Red and White PIWI ("Pilzwiderstandsfähig") Grapevines Possessing QTLs for Partial Resistance to Downy and Powdery Mildews.

Plants (Basel, Switzerland)·2024

Related Experiment Video

Updated: Jun 8, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.6K

How Helpful May Be a CRISPR/Cas-Based System for Food Traceability?

Silvia Farinati1, Aurélien Devillars1, Giovanni Gabelli1

  • 1Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), Campus of Agripolis, University of Padova, Viale dell'Università 16, 35020 Legnaro, Italy.

Foods (Basel, Switzerland)
|November 9, 2024
PubMed
Summary

Genome editing (GE) technologies, especially CRISPR/Cas, are revolutionizing crop breeding and biotechnology. This review highlights GE

Keywords:
CRISPR/Cas systemgenome editingmolecular traceabilitynew breeding techniquesnew genetic techniques

More Related Videos

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

150
High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
12:59

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

17.9K

Related Experiment Videos

Last Updated: Jun 8, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.6K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

150
High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
12:59

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

17.9K

Area of Science:

  • Agricultural Biotechnology
  • Molecular Biology
  • Food Science

Background:

  • Genome editing (GE) technologies offer transformative potential for crop breeding and agricultural biotechnology.
  • GE tools enable precise, site-specific genomic modifications, positioning them as New Breeding Techniques (NBTs).
  • CRISPR/Cas technology is a highly sensitive, accurate, and reproducible GE platform with broad applications in food crops.

Purpose of the Study:

  • To provide an overview of genome editing technologies in crop improvement.
  • To focus on the advances and non-editing applications of CRISPR/Cas systems.
  • To discuss the implications of these advances for molecular traceability and food safety.

Main Methods:

  • Review of existing literature on genome editing technologies, particularly CRISPR/Cas.
  • Analysis of CRISPR/Cas applications beyond traditional gene editing, including imaging, gene regulation, and detection.
  • Discussion of the impact of GE on molecular traceability and food safety.

Main Results:

  • CRISPR/Cas technology represents a significant advancement over previous biotech techniques for targeted gene editing in plants.
  • Emerging non-editing applications of CRISPR/Cas systems are expanding, including cell imaging, gene expression regulation, and chemical detection.
  • These GE applications have profound implications for molecular traceability, a critical aspect of food safety.

Conclusions:

  • Genome editing, particularly CRISPR/Cas, is a powerful tool for advancing agriculture and biotechnology.
  • The non-editing applications of CRISPR/Cas systems are rapidly evolving, offering novel solutions in various fields.
  • Enhanced molecular traceability through GE technologies is crucial for ensuring food safety and public trust.