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QSOX1 Modulates Glioblastoma Cell Proliferation and Migration In Vitro and Invasion In Vivo.

Reetika Dutt1, Colin Thorpe1, Deni S Galileo2

  • 1Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.

Cancers
|November 9, 2024
PubMed
Summary

Reducing Quiescin Sulfhydryl Oxidase 1 (QSOX1) in glioblastoma cells slows proliferation and invasion. This enzyme

Keywords:
QSOX1chick embryo brainglioblastomalentivirusshRNAxenograft

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Area of Science:

  • Biochemistry
  • Cancer Biology
  • Cell Biology

Background:

  • Quiescin Sulfhydryl Oxidase 1 (QSOX1) catalyzes thiol oxidation, forming disulfide bonds crucial for protein function.
  • QSOX1 is upregulated in various cancers, correlating with aggressive disease and poor prognosis.
  • Glioblastoma (GBM) is highly invasive and difficult to treat, necessitating research into invasion mechanisms.

Purpose of the Study:

  • To investigate the role of QSOX1 in human glioblastoma (GBM) cell invasion.
  • To determine if QSOX1 expression influences GBM cell proliferation, motility, and invasiveness.

Main Methods:

  • Utilized two T98G-derived glioblastoma cell lines.
  • Reduced QSOX1 expression using lentiviral shRNA, confirmed by Western blot.
  • Assessed proliferation (flow cytometry), in vitro motility (SuperScratch, Transwell assays), and in vivo invasiveness (chick embryo xenograft model).

Main Results:

  • QSOX1 knockdown led to a 27% reduction in S-phase cells, indicating decreased proliferation.
  • SuperScratch assay showed a 45% decrease in migration velocity in QSOX1-knockdown cells, with partial rescue via paracrine effects.
  • Transwell migration assay showed increased motility in knockdown cells, while chick embryo xenografts revealed reduced in vivo invasiveness.

Conclusions:

  • Experimental reduction of QSOX1 in GBM cells decreases proliferation.
  • QSOX1 knockdown alters in vitro cell migration patterns.
  • Reduced QSOX1 expression significantly decreases glioblastoma cell invasion in vivo.