Related Experiment Video

Updated: Jun 7, 2025

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.3K

SNHG15 Mediates MTSS1 Gene Expression via Interacting with the Gene Promoter and Regulating Transcription Pausing

Yanchun Zhou1, Shaoying Chen1, Weibin Chen1

  • 1Key Immunopathology Laboratory of Guangdong Province, Department of Pathophysiology, Shantou University Medical College, Shantou 515041, China.

International Journal of Molecular Sciences
|November 9, 2024
PubMed

Insights

Long non-coding RNA SNHG15 suppresses metastasis suppressor 1 (MTSS1) expression in breast cancer. SNHG15 complexes with proteins to block MTSS1 transcription, promoting cancer cell invasion and proliferation.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Gene Regulation

Background:

  • Metastasis suppressor 1 (MTSS1) is crucial for inhibiting cancer progression.
  • Understanding the regulation of MTSS1 expression is vital for developing cancer therapies.

Purpose of the Study:

  • To elucidate the mechanism controlling MTSS1 gene expression.
  • To investigate the role of long non-coding RNA SNHG15 in MTSS1 regulation.

Main Methods:

  • Investigated SNHG15 effects on MTSS1 mRNA levels in breast cancer cells.
  • Analyzed SNHG15 interaction with the MTSS1 promoter region.
  • Utilized co-immunoprecipitation to identify protein complexes involving SNHG15, DDX5, and RTF1.

Main Results:

  • SNHG15 expression inversely correlated with MTSS1 mRNA levels, increasing cell invasion and proliferation.
  • SNHG15 directly repressed MTSS1 promoter activity.
  • SNHG15, complexed with DDX5 and RTF1, interferes with RNA Polymerase II initiation at the MTSS1 core promoter, inducing transcriptional pausing.

Conclusions:

  • SNHG15 acts as a molecular regulator suppressing MTSS1 transcription.
  • The SNHG15-DDX5-RTF1 complex inhibits MTSS1 gene expression, potentially driving breast cancer progression.
Keywords:
LncRNA SNHG15MTSS1transcriptional regulation

More Related Videos

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

1.9K
Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

2.0K

Related Concept Videos

Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
1
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.6K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.9K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K