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Updated: May 11, 2026

Video-oculography in Mice
Published on: July 19, 2012
Eyelid Motion Tracking During Blinking Using High-Speed Imaging and Digital Image Correlation
Andrew Seamone1, Jeremy N Shapiro2,3, Zhenyang Zhao4
1Department of Aerospace Engineering, University of Michigan, Ann Arbor, MI 48105.
Abstract:
This study presents a novel technique to measure the motion of the eyelid during blinking. High-speed imaging and digital image correlation (DIC) were employed to monitor the eyelid during blinking in a noninvasive manner. Both spontaneous and reflex blinks were studied. A black liquid eyeliner was used to generate a speckle pattern on the surface of the eyelid. Facet motion captured through a DIC analysis software generated kinematic data for each blink. Calculations using this dataset yielded information on the duration of the blink, eyelid displacements, and peak eyelid velocities. A consistent dataset quantified the difference between blink types and reinforced the repeatability of this DIC analysis method to measure the kinematics of blinking.
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