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Updated: Aug 9, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
A facile and selective resonance Rayleigh scattering method for trace nitrite using gold nanocluster surface
Ruitao Zhu1,2,3, Zhiqiang Wang1,2,3, Aihui Liang1,2,3
1Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin, 541004, China.
Abstract:
A novel gold nanocluster surface molecularly imprinted polyisopropylacrylamide probe (AuNC@MIP) was synthesized for the specific detection of NO2-, using N-isopropylacrylamide (NIPAM) as the functional monomer, gold nanoclusters (AuNC) as the substrate, ethylene glycol dimethacrylate (EGDMA) as the cross-linking agent, and nitrosophenol (NPN) produced from sodium nitrite (NaNO2) and phenol (PN) as the template molecule, N,N-dimethylformamide (DMF) as the solvent, and azobisisobutyronitrile (AIBN) as the initiator. The nanoprobe was characterized using molecular spectroscopy, XPS, TEM, TGA, and zeta potential analysis. The probes revealed a prominent resonance Rayleigh scattering (RRS) peak at 370 nm. Upon addition of NO2-, the RRS intensity at 370 nm decreased due to the RRS energy transfer enhancing. The linear determination range is 0.125-1.50 nmol/L NO2-, with a limit of detection (LOD) of 0.085 nmol/L. The new RRS method was applied to determine NO2- in meat, dairy products, and water samples, with recovery of 96-107% also relative standard deviations (RSDs) of 1.1-8.0%.
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