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The activation of SYNJ2/GRB2 axis accelerates the malignant metastasis and angiogenesis of gastric cancer cells

  • 0Department of General Surgery, Digestive Disease Hospital, Affiliated Hospital of Zunyi Medical University, China.
Molecular and Cellular Probes +

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November 9, 2024

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November 9, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Synaptojanin 2 (SYNJ2) promotes gastric cancer (GC) metastasis and angiogenesis by increasing growth factor receptor-bound protein 2 (GRB2) levels. Targeting SYNJ2 may inhibit GC progression and metastasis.

Area Of Science

  • Oncology
  • Molecular Biology
  • Cancer Research

Background

  • Gastric cancer (GC) metastasis to lymph nodes is a critical factor in patient prognosis.
  • Synaptojanin 2 (SYNJ2) has been implicated in the progression of various cancers, but its specific role in GC metastasis remains unclear.

Purpose Of The Study

  • To elucidate the underlying mechanism by which SYNJ2 influences gastric cancer progression, focusing on its role in metastasis and angiogenesis.
  • To investigate the relationship between SYNJ2 and growth factor receptor-bound protein 2 (GRB2) in GC cells.

Main Methods

  • SYNJ2 expression analysis in GC tissues using the GEPIA database.
  • In vitro assays including cell transfection (shSYNJ2, shGRB2, SYNJ2 overexpression, GRB2 overexpression), qRT-PCR, CCK-8, flow cytometry, wound healing, Transwell invasion, and tube formation assays.
  • Western blot analysis for protein expression and Co-immunoprecipitation (CO-IP) assay to assess protein interactions.

Main Results

  • SYNJ2 was found to be highly expressed in GC tissues and cells.
  • SYNJ2 overexpression enhanced GC cell viability, migration, invasion, and angiogenesis, while inhibiting apoptosis. Conversely, SYNJ2 knockdown showed opposite effects.
  • SYNJ2 was shown to interact with GRB2, and GRB2 played a crucial role in mediating the effects of SYNJ2 on cell migration, invasion, angiogenesis, and metastasis-related protein levels.

Conclusions

  • SYNJ2 promotes gastric cancer cell metastasis and angiogenesis.
  • The mechanism involves the up-regulation of GRB2 by SYNJ2.
  • SYNJ2 represents a potential therapeutic target for inhibiting GC progression and metastasis.

Keywords:

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