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Novel thermal stability enhanced xylanase improves the performance and digestibility parameters in broilers.
Su Rin Lee1, Daulat Rehman Khan2, Jae Yong Park3
1Institute of Biotechnology, CJ CheilJedang Co., Suwon 16495, Republic of Korea.
Poultry Science
|November 10, 2024
Summary
Engineered xylanase OXynA-M demonstrates enhanced thermal and pH stability, improving broiler chicken performance by reducing digesta viscosity and increasing nutrient digestibility. This enzyme shows promise for effective use in animal feed applications.
Area of Science:
- Biotechnology and Enzyme Engineering
- Animal Nutrition and Feed Science
Background:
- Xylanases are crucial in animal feed but require thermal and pH stability, along with resistance to inhibitors, for optimal efficacy.
- A GH11 xylanase from Orpinomyces sp. strain PC-2 exhibits intrinsic high specific activity and inhibitor resistance.
- Rational protein design was employed to enhance the enzyme's stability for industrial applications.
Purpose of the Study:
- To engineer a highly stable and effective xylanase (OXynA-M) for animal feed applications.
- To evaluate the in vitro properties and in vivo efficacy of the engineered xylanase in broiler chickens.
Main Methods:
- Rational protein design was used to create OXynA-M from a native GH11 xylanase.
- In vitro characterization included thermal stability (differential scanning calorimetry), pH stability, and inhibitor resistance assays.
- An in vivo trial with 600 broiler chickens assessed performance parameters, digesta viscosity, nutrient digestibility, and apparent metabolizable energy (AME_n) across varying enzyme dosages.
Main Results:
- OXynA-M exhibited a melting temperature of 87.2°C and stability across pH 2.0-10.0.
- The enzyme demonstrated resistance to TAXI-IB, TAXI-IIA, and XIP xylanase inhibitors.
- In broiler chickens, OXynA-M supplementation dose-dependently improved performance, reduced ileal digesta viscosity (down to 6.54 cP at 1200 U/kg), enhanced crude protein, fat, and starch digestibility, and increased AME_n (notably at 9600 U/kg).
Conclusions:
- The engineered OXynA-M xylanase possesses superior thermal and pH stability and inhibitor resistance, making it suitable for animal feed.
- In vivo studies confirm OXynA-M's efficacy in improving broiler chicken growth performance, nutrient utilization, and energy value of feed.
- OXynA-M represents a valuable tool for enhancing the nutritional value and efficiency of animal feed formulations.
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