Achieving efficient anammox contribution and the enrichment of functional bacteria in partial denitrification/anammox
Zilong Hou1, Wenyi Dong2, Yanchen Li1
1School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen 518055, China.
Abstract:
The primary challenge of applying partial denitrification/anammox (PD/A) to municipal wastewater treatment lied in the enrichment of functional bacteria with a considerable autotrophic nitrogen removal performance. The results showed influent NO3--N: NH4+-N, reaction time and temperature would influence anammox nitrogen removal contribution. 15N isotopic tracing technology further revealed the average anammox contribution rate was up to 94.8 %. Extending reaction time was an effective measure to improve simultaneously PD and anammox activity. Microbial community indicated partial denitrifying bacteria (Bacillus) and anammox bacteria (Candidatus Brocadia) were enriched with abundance of 27.27 % and 7.09 % at NO3--N: NH4+-N of 1:1. The correlation analysis showed that NO3--N: NH4+-N ratio played the positive role for Bacillus enrichment, and low temperature was favorable to the enrichment of Thauera and Candidatus Jettenia. Overall, this study demonstrated the reasonable operational strategy would strengthen anammox contribution and facilitate enrichment of functional bacteria.
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