Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitigating Fixation Artifacts in Spatial Transcriptomics: Methodological Insights from Human Brain Tissue.

Molecular neurobiology·2026
Same author

Characteristics of child physical abuse cases that are referred by the police for a clinical forensic medical examination.

Medicine, science, and the law·2026
Same author

Quantitative Histological Insights Into Sudden Arrhythmic Death Syndrome: Findings From a Forensic Autopsy Cohort.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
Same author

Clinical forensic examinations and prosecution in Danish child physical abuse cases.

Child abuse & neglect·2025
Same author

[Christmas article: From holiday candy to carnival buns: A systematic mapping of cake consumption in a forensic daily routine].

Ugeskrift for laeger·2025
Same author

Characteristics of drug-facilitated sexual assault (DFSA): an observational study at a Danish sexual assault center.

International journal of legal medicine·2025

Related Experiment Video

Updated: Jun 7, 2025

Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles
10:30

Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles

Published on: October 15, 2014

20.5K

Quantifying Cardiac Tissue Composition Using QuPath and Cellpose: An Accessible Approach to Postmortem Diagnosis.

Pernille Heimdal Holm1, Kristine Boisen Olsen1, Richard Denis Maxime De Mets2

  • 1Section of Forensic Pathology, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|November 10, 2024
PubMed
Summary

An automated pipeline quantifies cardiac tissue changes postmortem, aiding diagnosis of arrhythmogenic cardiomyopathy. This method offers a free, reproducible template for unbiased evaluation of cardiac diseases.

Keywords:
arrhythmogenic cardiomyopathyautomatic quantificationmyocardial fatmyocardial fibrosispostmortem diagnosissudden cardiac death

More Related Videos

High-Resolution Cardiac Positron Emission Tomography/Computed Tomography for Small Animals
11:09

High-Resolution Cardiac Positron Emission Tomography/Computed Tomography for Small Animals

Published on: December 16, 2022

3.6K
Assessing Cardiac Reprogramming using High Content Imaging Analysis
06:02

Assessing Cardiac Reprogramming using High Content Imaging Analysis

Published on: October 26, 2020

1.2K

Related Experiment Videos

Last Updated: Jun 7, 2025

Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles
10:30

Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles

Published on: October 15, 2014

20.5K
High-Resolution Cardiac Positron Emission Tomography/Computed Tomography for Small Animals
11:09

High-Resolution Cardiac Positron Emission Tomography/Computed Tomography for Small Animals

Published on: December 16, 2022

3.6K
Assessing Cardiac Reprogramming using High Content Imaging Analysis
06:02

Assessing Cardiac Reprogramming using High Content Imaging Analysis

Published on: October 26, 2020

1.2K

Area of Science:

  • Forensic Pathology
  • Cardiovascular Pathology
  • Digital Pathology

Background:

  • Sudden cardiac death often presents as the first symptom of underlying heart disease.
  • Diagnosing arrhythmogenic cardiomyopathy postmortem is challenging due to variable structural changes.
  • Concealed cardiomyopathy, diagnosed by genotype, lacks definitive postmortem structural findings.

Purpose of the Study:

  • To develop and validate an automated digital pathology pipeline for quantifying postmortem cardiac tissue.
  • To assess myocardial fibrosis, residual myocardium, and adipocytes in postmortem cardiac samples.
  • To establish a reproducible method for unbiased postmortem cardiac disease evaluation.

Main Methods:

  • An automated pipeline using QuPath and Cellpose was developed for image analysis.
  • Picrosirius red staining was used to quantify collagen, myocardium, and adipocytes.
  • The pipeline was tested on cardiac tissues from autopsied individuals, including arrhythmogenic cardiomyopathy cases and controls.

Main Results:

  • The automated pipeline successfully quantified collagen, myocardium, and adipocytes in postmortem cardiac tissue.
  • This method provides a quantitative approach to analyzing reactive cardiac changes.
  • The developed pipeline is free, easy to implement, and serves as a template for other research.

Conclusions:

  • The automated pipeline offers a novel, unbiased method for postmortem cardiac tissue analysis.
  • This approach can aid in developing quantitative diagnostic criteria for postmortem cardiac diseases.
  • The method facilitates efficient evaluation of cardiac measurements, particularly for rare conditions like arrhythmogenic cardiomyopathy.