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  1. Home
  3. Heat Shock Protein 70-2 Is Overexpressed In Oral Leukoplakia And Oral Squamous Cell Carcinoma.
  1. Home
  3. Heat Shock Protein 70-2 Is Overexpressed In Oral Leukoplakia And Oral Squamous Cell Carcinoma.

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Heat Shock Protein 70-2 is Overexpressed in Oral Leukoplakia and Oral Squamous Cell Carcinoma.

Ran Li1, Xiaofeng Jiao1, Yixuan Gu1

  • 1Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Shanxi Medical University School and Hospital of Stomatology, Taiyuan, Shanxi, China; Department of Pediatric and Preventive Dentistry, Shanxi Medical University School and Hospital of Stomatology, Taiyuan, Shanxi, China.

International Dental Journal
|November 10, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Heat shock protein 70-2 (HSP70-2) is significantly expressed in oral potentially malignant disorders and oral squamous cell carcinoma. HSP70-2 plays a key role in oral cancer development and may serve as a therapeutic target.

Keywords:
Heat shock protein 70-2Oral leukoplakiaOral squamous cell carcinomaTumor biomarker

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Area of Science:

  • Oral oncology
  • Molecular biology
  • Biomarker discovery

Background:

  • Oral potentially malignant disorders (OPMD) and oral squamous cell carcinoma (OSCC) represent a significant global health challenge.
  • Understanding the molecular mechanisms underlying oral cancer development is crucial for early diagnosis and effective treatment.

Purpose of the Study:

  • To investigate the expression of heat shock protein 70-2 (HSP70-2) in OPMD and OSCC.
  • To elucidate the role of HSP70-2 in the pathogenesis of oral malignant diseases.
  • To assess HSP70-2 as a potential biomarker for oral cancer.

Main Methods:

  • Immunohistochemistry, RT-qPCR, Western blot, indirect immunofluorescence, and flow cytometry were employed to analyze HSP70-2 expression in tissue and cell samples.
  • In vitro studies involved knocking down HSP70-2 gene expression using liposomal vector transient transfection to assess its functional role.

Main Results:

  • HSP70-2 mRNA and protein were significantly expressed in OPMD and OSCC tissues and cell lines, with expression levels correlating with disease progression.
  • Downregulation of HSP70-2 inhibited proliferation, viability, colony formation, migration, and invasion of oral cancer cells.
  • HSP70-2 knockdown induced apoptosis and cell cycle arrest in OPMD and OSCC cells.

Conclusions:

  • HSP70-2 exhibits differential expression in normal, OPMD, and OSCC tissues, indicating its involvement in oral cancer development.
  • HSP70-2 is a promising biomarker for predicting malignant transformation of oral leukoplakia (OLK) and early OSCC diagnosis.
  • HSP70-2 represents a potential therapeutic target for early intervention in OLK and for siRNA-based treatment strategies in OSCC.