Influence of experimental conditions on some in-vitro biomechanical properties of the sow's perineum

The aim of this work was to develop an experimental protocol that takes into account the influence of experimental conditions on these perineal tissues, before determining their mechanical properties. Samples of each perineal tissue layer were obtained from the skin, the vagina, the external anal sphincter (EAS), the internal anal sphincter (IAS) and anal mucosa of freshly dead sows. They were tested in quasi-static uniaxial tension using the Mach-1 testing machine. Stress-strain curves of each perineal tissue layer before the first damage for each sow were obtained and modeled by hyperelastic laws described by three coefficients: C1, C2, and C3 (Yeoh model). The influence of sample preparation conditions such as tissue freezing, hygrometry and sample orientation were evaluated, and the conditions under which the tests were performed such as the displacement velocity during testing were also evaluated by analysing C1-coefficient. This study suggested that sample preparation conditions such as tissue freezing for 24 h, storage in cellophane paper for two hours and the strain rate did not statistically affect the C1-hyperelastic coefficient for each perineal layer (p > 0.05). Samples should not be stored in saline for 2 h (p < 0.05). Sample orientation did not influence C1-hyperelastic coefficient (p > 0.05). This experimental protocol could be used to study in vitro biomechanical properties of perineal tissues in order to better understand perineal tears during delivery.