Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transmission Electron Microscopy01:15

Transmission Electron Microscopy

5.4K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
5.4K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Role of Fetuin-A on the Attachment and Proliferation of Osteoblast-like Cells on Model Gold Surfaces.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Novel Hybrid Semiconductor-Cellular Standard for 3D FIB-SEM Nanotomography Analysis.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same author

Cross-linking of collagen fibrils leads to preferential gap zone mineralization in vitro.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Identification and characterization of a wet adhesive protein extracted from <i>Dreissena bugensis</i>, the freshwater quagga mussel.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Casein Phosphopeptides as a Model for Noncollagenous Protein Control of Collagen Mineralization <i>In Vitro</i>.

Biomacromolecules·2025
Same author

Real-Time Visualization of Calcium Phosphate Formation on Titanium Dioxide Nanoparticles Using Liquid Transmission Electron Microscopy.

Small (Weinheim an der Bergstrasse, Germany)·2025

Related Experiment Video

Updated: Jun 7, 2025

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy
07:37

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy

Published on: December 20, 2012

12.6K

Exploring Biomineralization Processes Using In Situ Liquid Transmission Electron Microscopy: A Review.

Liza-Anastasia DiCecco1,2, Tengteng Tang1,3, Eli D Sone4,5,6

  • 1Department of Materials Science and Engineering, McMaster University, Hamilton, ON, L8S 4L7, Canada.

Small (Weinheim an Der Bergstrasse, Germany)
|November 11, 2024
PubMed
Summary

Liquid transmission electron microscopy (TEM) offers real-time insights into biomineralization, revealing dynamic, non-classical pathways. This technique shifts understanding from static observations to mechanistic visualization in biological systems.

Keywords:
amorphous precursorsbiomineralization, biominerals, crystallizationliquid electron microscopy

More Related Videos

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm
15:39

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm

Published on: February 28, 2017

8.2K
In situ TEM of Biological Assemblies in Liquid
08:28

In situ TEM of Biological Assemblies in Liquid

Published on: December 30, 2013

10.1K

Related Experiment Videos

Last Updated: Jun 7, 2025

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy
07:37

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy

Published on: December 20, 2012

12.6K
Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm
15:39

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm

Published on: February 28, 2017

8.2K
In situ TEM of Biological Assemblies in Liquid
08:28

In situ TEM of Biological Assemblies in Liquid

Published on: December 30, 2013

10.1K

Area of Science:

  • Biomineralization research
  • Materials science
  • Microscopy techniques

Background:

  • Biomineralization theories traditionally focused on classical crystallization pathways.
  • Previous observations using traditional and cryo-TEM suggested non-traditional routes involving precursor phases.
  • Static imaging techniques limited the dynamic understanding of these complex processes.

Purpose of the Study:

  • To review recent in situ liquid transmission electron microscopy (TEM) research in biomineralization.
  • To highlight the shift towards non-classical biomineralization theories enabled by dynamic visualization.
  • To provide practical guidance on liquid TEM methods for researchers in the field.

Main Methods:

  • In situ liquid transmission electron microscopy (TEM) for real-time observation of reactions.
  • Dynamic mechanistic visualization of biomineralization processes.
  • Critical review of recent literature on liquid TEM applications in biomineralization.

Main Results:

  • Liquid TEM has revealed complex and multifaceted biomineralization processes with real-time resolution.
  • Dynamic observation of both classical and non-classical mineralization pathways, particularly for Ca and Fe systems.
  • Demonstrated the capability of liquid TEM to provide insights beyond static imaging limitations.

Conclusions:

  • Liquid TEM is a transformative technique for studying biomineralization, offering dynamic mechanistic insights.
  • It supports the paradigm shift towards non-classical theories by visualizing dynamic precursor phases.
  • Future expansion of liquid TEM research promises significant discoveries in understanding biological systems.