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Related Concept Videos

Cleavage and Blastulation01:33

Cleavage and Blastulation

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After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
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Related Experiment Video

Updated: May 3, 2026

Transcriptome Analysis of Single Cells
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Transcriptome Analysis of Single Cells

Published on: April 25, 2011

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A comparative analysis of blastoid models through single-cell transcriptomics.

Ali Balubaid1, Samhan Alsolami1, Narsis A Kiani2,3

  • 1Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Iscience
|November 11, 2024
PubMed
Summary
This summary is machine-generated.

Blastoid models derived from naive pluripotent stem cells (nPSCs) more closely resemble human blastocysts than those from extended pluripotent stem cells (EPSCs). This highlights how starting cell type heterogeneity impacts early human embryogenesis models.

Keywords:
Developmental biologyStem cells researchTranscriptomics

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Reproductive Biology

Background:

  • Pluripotent stem cell-derived blastocyst-like structures (blastoids) are valuable models for studying early human embryogenesis (5-10 days post-fertilization).
  • The precise similarity of current blastoid models to natural human blastocysts remains an area of active investigation.
  • Understanding these similarities is crucial for refining blastoid models for research.

Purpose of the Study:

  • To compare the developmental fidelity of blastoids generated from different types of pluripotent stem cells to natural human blastocysts.
  • To investigate how the heterogeneity of starting pluripotent stem cell lines influences blastoid differentiation and lineage commitment.
  • To identify key factors for optimizing blastoid models for studying human embryogenesis.

Main Methods:

  • Comparative analysis of single-cell RNA sequencing (scRNAseq) data from seven blastoid models and human peri-implantation blastocysts.
  • Quantification of cell-type composition, transcriptomic overlap, and lineage-specific gene expression profiles.
  • Assessment of developmental propensities for primary (epiblast, primitive endoderm, trophectoderm) and secondary lineages (amnion, trophoblasts).

Main Results:

  • Blastoids derived from naive pluripotent stem cells (nPSCs) showed greater similarity to natural blastocysts compared to those from extended pluripotent stem cells (EPSCs).
  • EPSC-derived blastoids exhibited a higher proportion of primitive endoderm cells and ambiguous cells with endoderm signatures.
  • Analysis of starting cell lines revealed distinct transcriptomic heterogeneity in nPSCs and prevalent amnionic signatures in EPSCs, influencing blastoid outcomes.

Conclusions:

  • The heterogeneity of gene expression within founding pluripotent stem cell lines significantly impacts blastoid lineage differentiation.
  • Naive pluripotent stem cell-derived blastoids represent more accurate models of early human embryogenesis than EPSC-derived counterparts.
  • These findings provide critical insights for optimizing protocols to generate improved human embryogenesis models using blastoids.