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Neuroprotective Effect of Benzyl Ferulate on Ischemia/Reperfusion Injury via Regulating NOX2 and NOX4 in Rats: A Potential Antioxidant for CI/R Injury

  • 0Chest Pain Center of Changsha, The Affiliated Changsha Hospital of Hunan Normal University, Changsha 410006, Hunan, China.
Advances in Pharmacological and Pharmaceutical Sciences +

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November 11, 2024

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November 11, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Benzyl ferulate shows promise as an antioxidant for treating cerebral ischemia/reperfusion (CI/R) injury. It mitigates oxidative stress by modulating specific microRNAs and NADPH oxidase enzymes, reducing neuronal damage and apoptosis.

Area Of Science

  • Neuroscience
  • Biochemistry
  • Pharmacology

Background

  • Oxidative stress is a key factor in cerebral ischemia/reperfusion (CI/R) injury.
  • Antioxidants are vital for managing CI/R injury.
  • Benzyl ferulate's potential as an antioxidant requires further investigation.

Purpose Of The Study

  • To investigate the antioxidant effects of benzyl ferulate on CI/R injury.
  • To elucidate the underlying mechanisms of benzyl ferulate's action.
  • To evaluate benzyl ferulate's therapeutic potential for CI/R injury.

Main Methods

  • Established in vivo CI/R and in vitro hypoxia/reoxygenation (H/R) models.
  • Treated CI/R rats and SH-SY5Y cells with benzyl ferulate.
  • Assessed neurobiological function, infarct volume, reactive oxygen species (ROS), apoptosis, and specific molecular markers (miRNAs, NOX enzymes, SOD, MDA, cleaved caspase-3, Bax).

Main Results

  • Benzyl ferulate downregulated NADPH oxidase 2 (NOX2)/NOX4 and upregulated miRNAs (652/532/92b).
  • It reduced NOX enzyme activity, increased superoxide dismutase (SOD) activity, and decreased ROS and malondialdehyde (MDA) levels.
  • Inhibited apoptosis by reducing cleaved caspase-3 and Bax expression, mitigating CI/R and H/R injury.

Conclusions

  • Benzyl ferulate effectively mitigates oxidative stress in CI/R and H/R injury models.
  • Its mechanism involves modulating the miRNAs (652/532/92b)/NOX2/4 axis.
  • Benzyl ferulate is a promising antioxidant for treating CI/R injury.

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