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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Targeted Cancer Therapies02:57

Targeted Cancer Therapies

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The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
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Gene Therapy00:59

Gene Therapy

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Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
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Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

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Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
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What is Genetic Engineering?00:49

What is Genetic Engineering?

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Overview
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: Jun 7, 2025

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
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Emerging Gene-editing nano-therapeutics for Cancer.

Najma Nujoom1, Manzoor Koyakutty1, Lalitha Biswas1

  • 1Amrita School of Nanosciences and Molecular Medicine, Amrita Vishwavidyapeetham (University), Ponekkara P.O., Kochi, India.

Heliyon
|November 11, 2024
PubMed
Summary

CRISPR gene editing has advanced cancer therapy through precise genetic modifications and improved delivery systems. This review highlights novel techniques and nanoparticle delivery for enhanced anticancer applications.

Keywords:
Alternatives of CRISPR/Cas9CRISPR/Cas9CancerGene-editingNanoparticlesNon-viral delivery

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Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?
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CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
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CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
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CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
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CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • CRISPR/Cas9, discovered in 2012, revolutionized genome engineering with its simplicity and specificity.
  • Nobel Prize-winning technology enabling targeted DNA alteration, gene regulation, and epigenetic modifications.
  • Limitations of early CRISPR/Cas9 spurred development of modified systems and alternative gene-editing tools.

Purpose of the Study:

  • To review novel gene-editing techniques beyond the original CRISPR/Cas9 system.
  • To discuss recent advancements in nanoparticle-based delivery of CRISPR/Cas9 for cancer therapy.
  • To explore the application of gene editing in oncogene knockout, tumor suppressor gene repair, and CAR-T cell development.

Main Methods:

  • Review of recent scientific literature on gene-editing technologies and their applications in cancer.
  • Analysis of modifications to CRISPR/Cas9, including miniature-Cas proteins and alternative methods like OMEGA and Fanzor.
  • Examination of nanoparticle-based delivery systems for CRISPR/Cas9 components (guide RNA and Cas9).

Main Results:

  • CRISPR/Cas9 and its variants offer powerful tools for cancer research and therapy, including oncogene inactivation and tumor suppressor gene correction.
  • Development of novel gene-editing tools and strategies addresses limitations of the original CRISPR/Cas9 system.
  • Nanoparticle delivery systems are enhancing the tumor-specific application and clinical translation of CRISPR/Cas9-based cancer therapies.

Conclusions:

  • Gene editing holds significant promise for developing innovative anticancer therapies.
  • Continued advancements in gene-editing technologies and delivery methods are crucial for realizing the full potential of CRISPR in oncology.
  • Nanoparticle-mediated delivery is a key strategy for improving the efficacy and safety of CRISPR-based cancer treatments.