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Related Concept Videos

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Related Experiment Video

Updated: Jun 7, 2025

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
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A clenbuterol detection method based on magnetic separation up-conversion fluorescent probe.

Xin-Jie Song1, Fei Ye1, Yao Zhang1

  • 1Zhejiang Provincial Key Laboratory of Chemical and Biological Processing Technology for Agricultural Products, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Liuxia Street Number 318, Hangzhou 310023, PR China.

Food Chemistry: X
|November 11, 2024
PubMed
Summary

A new fluorescence detection method uses aptamer-modified nanoparticles to accurately quantify Clenbuterol (CLB) residues in pork. This sensitive assay offers a limit of detection of 0.304 ng/mL for reliable food safety analysis.

Keywords:
AptamerClenbuterolDetection methodMagnetic nanoparticlesUp-conversion nanoparticles

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Area of Science:

  • Analytical Chemistry
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Clenbuterol (CLB) is a banned substance in food production due to health risks.
  • Accurate and sensitive detection methods for CLB residues in food are crucial for public health and regulatory compliance.

Purpose of the Study:

  • To develop a novel fluorescence detection method for sensitive and accurate quantification of Clenbuterol (CLB).
  • To utilize aptamer-modified up-conversion nanoparticles (UCNPs) and magnetic nanoparticles (MNPs) for CLB detection.

Main Methods:

  • A sandwich assay was designed using aptamer-functionalized UCNPs and MNPs to capture and detect CLB.
  • The MNP-CLB-UCNP complex was isolated using a magnetic field, followed by fluorescence intensity measurement.
  • Spectrophotometry was employed with specific excitation (980 nm) and emission (400-800 nm) spectra.

Main Results:

  • The fluorescence intensity showed a direct correlation with increasing CLB concentrations, indicating good specificity.
  • The method achieved a high sensitivity with a limit of detection of 0.304 ng/mL for CLB.
  • Recovery rates for CLB in pork samples ranged from 84% to 94.87%, demonstrating accuracy.

Conclusions:

  • The developed fluorescence method provides a sensitive, precise, and accurate approach for quantifying CLB residues in food matrices like pork.
  • This aptamer-based nanoparticle assay offers a promising tool for food safety monitoring and control of banned substances.