PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing...

Area of Science:

• Genomics
• Bioinformatics
• Molecular Biology

Background:

• High-throughput transposon insertion sequencing (TIS) data analysis is complex and time-consuming.
• Existing methods often require significant bioinformatics expertise and produce static visualizations.
• Lack of accessible tools hinders real-time data interrogation and understanding of gene function.

Purpose of the Study:

• To develop an accessible web-based tool for visualizing and analyzing TIS data.
• To enable researchers of all bioinformatics skill levels to interrogate TIS datasets.
• To facilitate the identification of genes contributing to fitness in experimental systems.

Main Methods:

• Creation of PIMMS-Dash, a web-based browser tool.
• Integration with the PIMMS analysis pipeline for TIS data processing.
• Implementation of interactive and sortable tables for data exploration.

Main Results:

• PIMMS-Dash provides interactive visualization of TIS data.
• The tool allows for easy identification of genes of interest and their contribution to fitness.
• Researchers can generate publication-quality figures directly from the tool.

Conclusions:

• PIMMS-Dash democratizes TIS data analysis, making it accessible to a wider research community.
• The tool enhances understanding of gene fitness contributions through intuitive data interrogation.
• PIMMS-Dash supports efficient generation of high-quality figures for scientific publications.