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Updated: Jun 7, 2025

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

12.8K

MiR-301b-3p promotes breast cancer development through inhibiting the expression of transforming growth factor-beta

Jian Lou1, Xueni Liu1, Yanru Xie1

  • 1Tumor Center, Lishui Central Hospital, Lishui, China.

Peerj
|November 11, 2024
PubMed
Abstract

Insights

MicroRNA miR-301b-3p promotes breast cancer (BC) progression by enhancing cell growth, invasion, migration, and inhibiting apoptosis. Targeting transforming growth factor-beta receptor 2 (TGFBR2) counteracts these tumor-promoting effects.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Breast cancer (BC) remains a significant health concern.
  • Understanding the molecular mechanisms driving BC progression is crucial for developing effective therapies.

Purpose of the Study:

  • To investigate the role of miR-301b-3p in breast cancer.
  • To elucidate the mechanism involving miR-301b-3p and transforming growth factor-beta receptor 2 (TGFBR2) in BC progression.

Main Methods:

  • Cell culture of BC and normal breast epithelial cells.
  • Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
  • Cell counting kit-8 (CCK-8), Transwell, and wound healing assays for cell viability, invasion, and migration.
  • Flow cytometry for apoptosis analysis and Western blot for STAT protein expression.

Main Results:

  • miR-301b-3p was highly expressed in BC cells, while TGFBR2 was inhibited.
  • Inhibition of miR-301b-3p suppressed BC cell viability, invasion, and migration, effects reversed by TGFBR2 inhibition.
  • miR-301b-3p promoted anti-apoptosis, whereas TGFBR2 promoted apoptosis in BC cells.
  • miR-301b-3p influenced STAT1 and STAT3 phosphorylation, promoting BC progression.

Conclusions:

  • miR-301b-3p significantly promotes BC cell growth, invasion, migration, and anti-apoptosis.
  • Targeting TGFBR2 can effectively inhibit the tumor-promoting effects of miR-301b-3p in breast cancer.
Keywords:
Antagonism of miR-301b-3p and TGFBR2Breast cancerMiR-301b-3p-inhibitorTransforming growth factor-beta receptor 2 (TGFBR2)Transwell assay

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