Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bone Marrow Sampling and Transplants

Bone Marrow Sampling and Transplants

Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy the...

Cells of the Adaptive Immune Response

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Immunomodulatory and cellular effects of Aeromonas salmonicida melanin in Atlantic salmon (Salmo salar).

Fish & shellfish immunology·2026

Same author

Expression of Estrogen Receptor Alpha and Ki-67 in Synchronous Canine Mammary Tumors.

Journal of mammary gland biology and neoplasia·2026

Same author

Transcriptomic Profiling of Canine Mammary Tumours Reveals Significant Heterogeneity Between and Within Histological Classes.

Veterinary and comparative oncology·2026

Same author

Melanized Focal Changes in the Fillets of Farmed Atlantic Salmon: A Clinical Report From a Deep-Sea Production Site.

Journal of fish diseases·2026

Same author

Ecotoxicological effects of short-term exposure to elongated tunnel particles from two road construction sites on juvenile Atlantic salmon (Salmo salar).

Environmental research·2025

Same author

Transitional lumbosacral vertebrae in black Norwegian elkhound and Brittany dogs: Clinical findings and its association with degenerative lumbosacral stenosis.

Acta veterinaria Scandinavica·2025

Search research articles

Related Experiment Video

Updated: Jul 18, 2026

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

Immunological Pre-Metastatic Niche in Dogs With Naturally Occurring Osteosarcoma.

Mikael Kerboeuf¹, Kristin Paaske Anfinsen², Erling Olaf Koppang¹

¹Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, Norway.

Veterinary and Comparative Oncology

|November 11, 2024

Summary

Pre-metastatic niche formation in dogs with osteosarcoma (OS) shows increased bone marrow-derived cells in the lungs before metastasis. This canine model offers insights for developing therapies targeting pre-metastatic niches.

Keywords:

bone cancer canine metastasis myeloid cells osteosarcoma

More Related Videos

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

Author Spotlight: Replicating Human Osteosarcoma Progression in Immunodeficient Mice for Cancer Study

Author Spotlight: Replicating Human Osteosarcoma Progression in Immunodeficient Mice for Cancer Study

Published on: March 22, 2024

Related Experiment Videos

Last Updated: Jul 18, 2026

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

Author Spotlight: Replicating Human Osteosarcoma Progression in Immunodeficient Mice for Cancer Study

Author Spotlight: Replicating Human Osteosarcoma Progression in Immunodeficient Mice for Cancer Study

Published on: March 22, 2024

Area of Science:

Oncology
Veterinary Medicine
Cell Biology

Background:

Pre-metastatic niche (PMN) formation is a critical step in cancer metastasis, involving the remodeling of distant organ microenvironments by tumor-derived factors.
Osteosarcoma (OS) in dogs serves as a valuable translational model for human OS due to shared biological characteristics.

Purpose of the Study:

To investigate the presence and characteristics of pre-metastatic niches in the lungs of dogs with naturally occurring osteosarcoma.
To determine if bone marrow-derived cells (BMDCs) are recruited to the lungs prior to overt metastasis in this canine model.

Main Methods:

Multiplex immunofluorescence staining was used to quantify immune cell populations, including macrophages and monocytes, in lung tissues from treatment-naïve dogs with OS and healthy controls.
Lung tissues were analyzed from dogs with OS, categorized into those with and without lung metastases, and compared to control dogs.

Main Results:

Significantly higher numbers of CD204+ macrophages, CD206+ macrophages, monocytes, and CD11d+ bone marrow-derived cells (BMDCs) were observed in the lungs of dogs with OS compared to controls.
Increased total nucleated cell density was noted in the lungs of dogs with OS before metastasis.
CD11d+ BMDC numbers decreased in dogs with established lung metastases, suggesting transient recruitment.

Conclusions:

This study provides evidence for the existence of pre-metastatic niches in a naturally occurring canine osteosarcoma model.
Bone marrow-derived cells are recruited to the lungs preceding metastasis development in dogs with OS.
Canine osteosarcoma models are suitable for evaluating novel therapies targeting pre-metastatic niches.