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Fibrillar Hydrogel Inducing Cell Mechanotransduction for Tissue Engineering
Viktoriia V Egorova1, Mariia P Lavrenteva1, Liubov N Makhaeva2
1ChemBioCluster, ITMO University, Saint Petersburg 191002, Russian Federation.
Biomacromolecules
|November 11, 2024
Summary
Researchers developed a new hydrogel (MyoColl) for tissue engineering. This printable and cytocompatible material supports cell growth and shows potential for muscle tissue regeneration.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Bioprinting
Background:
- Designing multifunctional bioinks is crucial for tissue engineering, requiring a balance between printability and cytocompatibility.
- Current bioinks often face limitations in achieving optimal printability and cellular support simultaneously.
Purpose of the Study:
- To develop a novel fibrillar hydrogel, MyoColl, by combining B-type gelatin and oxidized sodium alginate via Schiff base formation, incorporating type I collagen.
- To evaluate the printability, cytocompatibility, and potential for muscle tissue engineering applications of the developed MyoColl hydrogel.
Main Methods:
- Schiff base formation between B-type gelatin and oxidized sodium alginate.
- Incorporation of type I collagen to form the MyoColl hydrogel.
- Characterization of hydrogel properties including sol-gel transition, shear thinning, Young's modulus, and shape accuracy.
- Assessment of cytocompatibility using metabolic activity tests and fluorescent microscopy of C2C12 myoblast cell cultures.
- Evaluation of cell mechanotransduction and myofilament formation in 3D printed MyoColl constructs.
Main Results:
- The MyoColl hydrogel demonstrated temperature- and mass-ratio-dependent sol-gel transitions, with tunable properties based on component ratios.
- The hydrogel exhibited excellent printability characteristics, including shear thinning, yielding, and shape accuracy.
- Significant cytocompatibility was observed for C2C12 myoblasts cultured on 2D MyoColl hydrogels.
- Primary signs of cell mechanotransduction and myofilament formation were detected in 3D printed MyoColl constructs.
Conclusions:
- MyoColl is a versatile and cytocompatible hydrogel platform suitable for biofabrication.
- The developed hydrogel shows significant promise as a scaffold for muscle tissue engineering.
- Further research into MyoColl could advance the field of regenerative medicine and tissue repair.
