Circ_0001741 exerts as a tumor promoter in ovarian cancer through the regulation of miR-491-5p/PRSS8 axis
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View abstract on PubMed
Summary
This summary is machine-generated.Circular RNA (circRNA) circ_0001741 promotes ovarian cancer (OC) growth and metastasis by interacting with miR-491-5p and PRSS8. This pathway offers a potential molecular target for OC treatment.
Area Of Science
- Oncology
- Molecular Biology
- Genetics
Background
- Circular RNAs (circRNAs) are key regulators in ovarian cancer (OC).
- Circ_0001741 is highly expressed in OC and influences paclitaxel resistance.
- Understanding circ_0001741's mechanism is crucial for OC progression.
Purpose Of The Study
- To investigate the role of circ_0001741 in ovarian cancer.
- To elucidate the molecular mechanism of circ_0001741 in OC progression.
- To explore circ_0001741 as a potential therapeutic target for OC.
Main Methods
- Quantification of circ_0001741, miR-491-5p, and PRSS8 levels using qRT-PCR and Western blot.
- Assessment of OC cell proliferation, apoptosis, and metastasis via CCK-8, Edu, flow cytometry, and Transwell assays.
- Validation of RNA interactions using dual-luciferase reporter and RIP assays; in vivo assessment via xenograft assay.
Main Results
- Circ_0001741 expression is upregulated in OC tissues and cell lines.
- Circ_0001741 knockdown inhibits OC cell proliferation, metastasis, and enhances apoptosis.
- Circ_0001741 targets miR-491-5p, which in turn targets PRSS8, promoting OC progression.
Conclusions
- Circ_0001741 promotes OC cell growth and metastasis via the miR-491-5p/PRSS8 axis.
- This pathway represents a potential molecular target for ovarian cancer therapy.
- Circ_0001741 knockdown inhibits tumor growth in vivo.
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