Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Creatine Exposure in <i>Caenorhabditis elegans</i> Induces Physiological and Morphological Changes without Metabolic Conversion to Creatinine: A Novel HPLC Analysis.

ACS omega·2025

Same author

The Fourth Annual Symposium of the Midwest Aging Consortium.

The journals of gerontology. Series A, Biological sciences and medical sciences·2024

Same author

Catalogue of Somatic Mutations in Cancer Database and Structural Modeling Analysis of CYP2D6 Mutations in Human Cancers.

The Journal of pharmacology and experimental therapeutics·2024

Same author

Detection of driver mutations and genomic signatures in endometrial cancers using artificial intelligence algorithms.

PloS one·2024

Same author

Quality Control Method and Device for Producing Agarose Micropads.

microPublication biology·2024

Same author

Early-life caffeine exposure induces morphological changes and altered physiology in Caenorhabditiselegans.

Biochemical and biophysical research communications·2023

Search research articles

Related Experiment Video

Updated: Jun 7, 2025

Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays

Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays

Published on: April 26, 2024

Measuring Cell Dimensions in Fission Yeast Using Machine Learning.

Kylie Lawson¹, Shayne Skrtic¹, Martin Vo^1,2

¹Biology Department, Xavier University, Cincinnati, OH, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 11, 2024

Summary

Automated cell measurement using PhotoPhenosizer (PP) revealed that replication stress in fission yeast (Schizosaccharomyces pombe) leads to larger cell size during aging. This highlights PP

Keywords:

Automation pipeline Cell segmentation Fission yeast Measuring algorithm

More Related Videos

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast

Published on: January 23, 2012

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Published on: January 11, 2018

Related Experiment Videos

Last Updated: Jun 7, 2025

Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays

Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays

Published on: April 26, 2024

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast

Published on: January 23, 2012

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Published on: January 11, 2018

Area of Science:

Cell Biology
Genetics
Microscopy

Background:

Cell length in fission yeast (Schizosaccharomyces pombe) is a key indicator of cell cycle progression.
Assessing cell size changes is vital for understanding disruptions in cell cycle dynamics due to genomic or metabolic stress.

Purpose of the Study:

To introduce and utilize PhotoPhenosizer (PP), a machine learning tool, for automated cell measurement and dimensional analysis in S. pombe.
To investigate the impact of genomic instability on cell size dynamics during chronological aging using PP.

Main Methods:

Employed brightfield (BF) microscopy to image S. pombe cells.
Utilized an automated cell segmentation algorithm integrated into the PhotoPhenosizer (PP) tool for precise cell measurement.
Conducted a 12-day chronological lifespan assay to track cell dimension changes in wild-type and replication stress mutant strains.

Main Results:

PhotoPhenosizer (PP) effectively streamlined morphological measurements for large-scale image processing.
Replication stress mutants exhibited significantly larger cell sizes compared to wild-type cells during chronological aging in glucose media.
Observed cell size changes are consistent with the activation of DNA damage-induced cell morphology checkpoints.

Conclusions:

PhotoPhenosizer (PP) is a valuable tool for automated, large-scale cell dimension analysis in S. pombe research.
Genomic instability, specifically replication stress, directly influences cell size dynamics and morphology during aging in fission yeast.
This methodology facilitates the study of cellular responses to various stresses, aiding S. pombe molecular research.