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Related Experiment Video

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Microscopy of Fission Yeast Sexual Lifecycle
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ChIPmentation for Epigenomic Analysis in Fission Yeast.

Felix Selasi Dewornu1, Pin Tong1, Sito Torres-Garcia1,2

  • 1Wellcome Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh, UK.

Methods in Molecular Biology (Clifton, N.J.)
|November 11, 2024
PubMed
Summary

We present ChIPmentation, a streamlined method for analyzing histone modifications and DNA-binding proteins in fission yeast. This technique reduces sample input and preparation time, making chromatin studies more efficient.

Keywords:
CENP-ACnp1ChIP-SeqChIPmentationFission yeastHeterochromatinHistone modificationsNucleosomeProtein-DNA interactions

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Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Histone modifications and transcription factor-DNA interactions are crucial for gene regulation and chromosome dynamics.
  • Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) is a key technique for mapping these elements genome-wide.
  • Existing ChIP-Seq protocols can be resource-intensive, requiring significant sample input and hands-on time.

Purpose of the Study:

  • To adapt and optimize a ChIPmentation protocol for the fission yeast Schizosaccharomyces pombe.
  • To reduce sample input requirements and hands-on time for ChIP-Seq experiments.
  • To lower the overall cost of chromatin-associated factor and histone modification analysis.

Main Methods:

  • Adaptation of a ChIPmentation protocol for Schizosaccharomyces pombe.
  • Integration of Tn5 transposase-mediated tagmentation with chromatin immunoprecipitation.
  • Optimization for reduced sample input and streamlined workflow.

Main Results:

  • Successful implementation of ChIPmentation in fission yeast.
  • Demonstrated reduction in required sample input compared to traditional ChIP-Seq.
  • Significant decrease in hands-on time and preparation costs.

Conclusions:

  • ChIPmentation offers a more efficient and cost-effective approach for studying epigenomic landscapes in Schizosaccharomyces pombe.
  • This optimized protocol facilitates broader accessibility to genome-wide analyses of histone modifications and DNA-binding proteins.
  • The method holds potential for advancing research in gene regulation and chromatin dynamics in yeast models.