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Related Experiment Video

Updated: Jun 26, 2026

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

Measuring Meiotic Recombination Frequency in Schizosaccharomyces pombe Using an Engineered Genetic Interval.

Alexander Lorenz¹

¹Institute of Medical Sciences (IMS), University of Aberdeen, Aberdeen, UK. a.lorenz@abdn.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)

|November 11, 2024

Summary

Fission yeast recombination assays track gene conversion and crossovers. New methods simultaneously measure spore viability and recombination events for comprehensive analysis.

Keywords:

Crossover Gene conversion Genetic interval Meiotic recombination Recombination hotspot Schizosaccharomyces pombe

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Last Updated: Jun 26, 2026

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Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Published on: October 11, 2022

Area of Science:

Molecular Biology
Genetics
Yeast Biology

Background:

Fission yeast (Schizosaccharomyces pombe) is a key model organism for studying meiosis.
Decades of research have utilized yeast to understand double-stranded DNA break (DSB) formation and repair during meiosis.
Genetic recombination assays are crucial for analyzing gene conversion and crossover frequencies.

Purpose of the Study:

To describe novel meiotic recombination assays in fission yeast.
To enable comprehensive assessment of reproductive success within a single assay.

Main Methods:

Engineered meiotic recombination assays using nutritional markers.
Markers were strategically placed upstream and downstream of the ade6 and ade7 genes.
Simultaneous measurement of spore viability, gene conversion, and crossover frequencies.

Main Results:

The developed assays allow for the simultaneous assessment of multiple meiotic parameters.
This provides a comprehensive evaluation of reproductive success in fission yeast.
Enables detailed investigation of cis and trans determinants of meiotic recombination.

Conclusions:

The described assays offer a powerful tool for studying meiotic recombination in Schizosaccharomyces pombe.
These assays facilitate a holistic understanding of recombination mechanisms and their impact on reproductive success.
This work advances the study of genetic recombination in a model eukaryotic system.