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Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Estimation of Scattering Properties Modifications Caused by In Vivo Human Skin Optical Clearing Using Line-Field

Sergey M Zaytsev¹, Léna Waszczuk^2,3, Jonas Ogien³

¹Université de Lorraine, CNRS, CRAN UMR 7039, Vandoeuvre-lès-Nancy, France.

Journal of Biophotonics

|November 11, 2024

Summary

Optical clearing reduces skin scattering, improving medical imaging depth and contrast. Researchers quantified these scattering changes in vivo using line-field confocal optical coherence tomography (LC-OCT).

Keywords:

modeling optical clearing optical coherence tomography optical properties scattering skin

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Published on: September 26, 2019

Area of Science:

Biomedical Optics
Dermatology
Medical Imaging

Background:

Optical methods for in vivo skin imaging are limited by light scattering.
Optical clearing techniques can reduce skin scattering, enhancing image contrast and probing depth.

Purpose of the Study:

To quantify in vivo modifications of skin scattering properties during optical clearing.
To evaluate the effectiveness of various optical clearing agents and enhancers on human skin.

Main Methods:

Utilized line-field confocal optical coherence tomography (LC-OCT) to acquire 3D images of human skin in vivo.
Applied nine mixtures of optical clearing agents with permeation enhancers.
Estimated scattering coefficient and anisotropy factor from in-depth intensity profiles using an exponential decay model.

Main Results:

Demonstrated a significant decrease in epidermal scattering, up to 33%, due to optical clearing.
Identified a specific mixture (polyethylene glycol, oleic acid, propylene glycol) yielding the best optical clearing results.
Quantified scattering property modifications in the epidermis and upper dermis.

Conclusions:

Optical clearing effectively reduces skin scattering in vivo.
LC-OCT is a suitable method for quantifying optical clearing effects on skin.
Optimized optical clearing formulations can significantly improve skin imaging parameters.