Marker rescue mapping of vaccinia virus temperature-sensitive mutants using overlapping cosmid clones representing...

A set of 11 overlapping cosmid clones of wild type (wt) vaccinia virus DNA was constructed. The clones together span almost the entire vaccinia virus genome. The clones were used to map temperature-sensitive (ts) mutants of vaccinia virus by marker rescue. Map positions were obtained for mutants representing 29 of 32 complementation groups tested.