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FOXN3 Regulates Autophagic Activity to Suppress Drug Resistance in Melanoma Cells

Yaqi Wang1,2, Hui Su1, Xiaopeng Wang1

  • 1Department of Dermatology, Xi'an Jiaotong University The Second Affiliated Hospital, Xi'an, Shaanxi, People's Republic of China.

Clinical, Cosmetic and Investigational Dermatology
|November 12, 2024
PubMed
Abstract

Insights

Forkhead box N3 (FOXN3) is downregulated in melanoma and inhibits cell proliferation and motility. FOXN3 targets Fibrous Sheath Interacting Protein 1 (FSIP1), making it a potential therapeutic target for drug-resistant melanoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Forkhead box N3 (FOXN3) is a transcriptional regulator implicated in tumor development.
  • Its specific role in melanoma pathogenesis and drug resistance remains largely uncharacterized.

Purpose of the Study:

  • To elucidate the biological functions of FOXN3 in the context of melanoma drug resistance.
  • To investigate FOXN3's role in melanoma cell proliferation, motility, and response to Vemurafenib.

Main Methods:

  • Gene expression analysis using GEPIA and Linkedomics databases.
  • In vitro assays including colony formation, scratch wound healing, and Transwell invasion.
  • In vivo xenograft studies in nude mice.
  • Chromatin immunoprecipitation and immunofluorescence to assess protein function and localization.

Main Results:

  • FOXN3 expression is downregulated in melanoma.
  • FOXN3 overexpression suppressed melanoma cell proliferation and motility, while knockdown enhanced them.
  • FOXN3 overexpression reduced autophagic activity; drug-resistant cells showed increased autophagy.
  • FOXN3 binds to the promoter of Fibrous Sheath Interacting Protein 1 (FSIP1), regulating its expression.

Conclusions:

  • FOXN3 is a key regulator in the development of Vemurafenib-resistant melanoma.
  • FOXN3's interaction with FSIP1 contributes to its role in drug resistance.
  • FOXN3 presents a promising therapeutic target for treating drug-resistant melanoma.
Keywords:
FOXN3Vemurafenibautophagydrug resistancemelanoma

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