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Structural and Functional Insight Into YefM-YoeB Complex of Toxin-Antitoxin System From Streptococcus pneumoniae

Do-Hee Kim1, Yong-Chan Lee2, Chenglong Jin2,3

  • 1Research Institute of Pharmaceutical Sciences, College of Pharmacy, Sookmyung Women's University, Seoul, Republic of Korea.

Journal of Cellular Biochemistry
|November 12, 2024
PubMed

Insights

The YefM-YoeB toxin-antitoxin system in Streptococcus pneumoniae was structurally and functionally characterized. Understanding this system offers potential for developing new antibiotics against drug-resistant bacterial infections.

Area of Science:

  • Microbiology
  • Structural Biology
  • Molecular Biology

Background:

  • Streptococcus pneumoniae causes severe diseases like pneumonia and meningitis.
  • Antibiotic resistance necessitates novel therapeutic mechanisms.
  • Toxin-antitoxin (TA) systems, like YefM-YoeB, are potential antimicrobial targets.

Purpose of the Study:

  • To elucidate the structural basis of the YefM-YoeB TA system in S. pneumoniae.
  • To investigate the functional activity of the YoeB toxin.
  • To understand the binding interactions for potential drug development.

Main Methods:

  • X-ray crystallography to determine the YefM-YoeB complex structure.
  • In vitro ribonuclease activity assays for YoeB toxin.
  • Analytical ultracentrifugation to determine the oligomeric state in solution.

Main Results:

  • The crystal structure of the S. pneumoniae YefM-YoeB complex was determined.
  • The ribonuclease activity of the YoeB toxin was confirmed.
  • The oligomeric state and a proposed DNA-binding mode of the complex were investigated.

Conclusions:

  • Structural and functional data provide insights into TA system mechanisms.
  • The YefM-YoeB system is a promising target for novel antibiotic development.
  • Targeting this system could combat antibiotic-resistant S. pneumoniae infections.
Keywords:
Streptococcus pneumoniaeX‐ray crystallographyYefM–YoeBantibioticstoxin–antitoxin system

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