Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional Inactivation of <i>PAX4</i> Results in Disrupted Endocrine Pancreas Development and Neonatal Diabetes in Pigs.

International journal of molecular sciences·2026
Same author

Characterization of A Bronchoscopically Induced Transgenic Lung Cancer Pig Model for Human Translatability.

bioRxiv : the preprint server for biology·2024
Same author

Pigs lacking <i>TMPRSS2</i> displayed fewer lung lesions and reduced inflammatory response when infected with influenza A virus.

Frontiers in genome editing·2024
Same author

Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas.

The CRISPR journal·2024
Same author

Development of Polarity-Reversed Endometrial Epithelial Organoids.

bioRxiv : the preprint server for biology·2023
Same author

Establishment, characterization, and validation of novel porcine embryonic fibroblasts as a potential source for genetic modification.

Frontiers in cell and developmental biology·2022

Related Experiment Video

Updated: Jun 7, 2025

Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine
09:54

Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine

Published on: November 4, 2018

8.1K

Blastocyst complementation generates exogenous donor-derived liver in ahepatic pigs.

Sean G Simpson1,2, Ki-Eun Park1,2, Sai Gautham Reddy Yeddula2

  • 1RenOVAte Biosciences Inc, Reisterstown, Maryland, USA.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|November 12, 2024
PubMed
Summary

Researchers created chimeric pig livers using a novel blastocyst complementation method. This breakthrough shows potential for generating donor-derived organs in large animals, addressing organ transplantation shortages.

Keywords:
CRE‐loxCRISPR/Casahepaticchimeragenome editingliverpigpluripotent cells

More Related Videos

Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model
12:49

Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model

Published on: August 17, 2022

2.4K
Technique of Porcine Liver Procurement and Orthotopic Transplantation using an Active Porto-Caval Shunt
12:27

Technique of Porcine Liver Procurement and Orthotopic Transplantation using an Active Porto-Caval Shunt

Published on: May 7, 2015

16.4K

Related Experiment Videos

Last Updated: Jun 7, 2025

Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine
09:54

Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine

Published on: November 4, 2018

8.1K
Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model
12:49

Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model

Published on: August 17, 2022

2.4K
Technique of Porcine Liver Procurement and Orthotopic Transplantation using an Active Porto-Caval Shunt
12:27

Technique of Porcine Liver Procurement and Orthotopic Transplantation using an Active Porto-Caval Shunt

Published on: May 7, 2015

16.4K

Area of Science:

  • Xenotransplantation
  • Organogenesis
  • Developmental Biology

Background:

  • Liver diseases cause significant global morbidity and mortality, with limited donor organs for transplantation.
  • Current organ transplantation faces challenges due to stringent criteria and organ scarcity.
  • Generating chimeric organs in pigs is a potential solution to bridge the organ shortfall.

Purpose of the Study:

  • To investigate the feasibility of generating chimeric livers in pigs.
  • To utilize a conditional blastocyst complementation approach for organogenesis.
  • To enable organ development using donor-derived pluripotent cells in a vacant niche.

Main Methods:

  • Sequential genetic modification of porcine fetal fibroblasts (FOXA3CREHHEXloxP/loxP).
  • Somatic cell nuclear transfer using a conditional HHEX knockout and GFP donor (COL1ACAG:LACZ 2A EGFP).
  • Generation of host embryos, complementation, and transfer into surrogate mothers.

Main Results:

  • Successful generation of chimeric pig fetuses.
  • Donor EGFP blastomeres were confirmed to reconstitute hepatocytes via immunohistochemistry.
  • Demonstrated feasibility of exogenous donor-derived hepatogenesis in a large animal model.

Conclusions:

  • The study demonstrates a viable approach for creating chimeric livers in pigs.
  • Conditional blastocyst complementation enables donor cell contribution to organ development.
  • This research paves the way for future advancements in regenerative medicine and organ transplantation.