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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

87.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Genomics02:02

Genomics

36.0K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Genome Annotation and Assembly03:36

Genome Annotation and Assembly

18.8K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

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Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
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Related Experiment Video

Updated: Jun 7, 2025

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

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Next-Generation Sequencing and Bioinformatics Consortium Approach to Genomic Surveillance.

Lindsay C Morton, Nazia Rahman, Kimberly A Bishop-Lilly

    Emerging Infectious Diseases
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    Summary
    This summary is machine-generated.

    Establishing robust genomic surveillance requires well-supported labs. The Global Emerging Infections Surveillance program

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    GEISGenomic surveillance. Global Emerging Infections SurveillanceNext-Generation Sequencing and Bioinformatics Consortiumbioinformaticsconsortiumsequencing

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    Area of Science:

    • Genomic epidemiology
    • Pathogen surveillance
    • Public health preparedness

    Background:

    • Genomic surveillance programs rely on dedicated, well-supported laboratories for pathogen monitoring.
    • The Global Emerging Infections Surveillance (GEIS) program offers valuable experience in developing and sustaining these capabilities.
    • Effective surveillance is crucial for public health and pandemic response.

    Purpose of the Study:

    • To outline a framework for building and maintaining next-generation sequencing (NGS) and bioinformatics capabilities for genomic surveillance within the US Department of Defense (DoD).
    • To leverage expertise in genome sequencing, bioinformatics, and genomic epidemiology to address the DoD and Military Health System's needs for pathogen monitoring.

    Main Methods:

    • Convening a consortium of experts in genome sequencing, bioinformatics, and genomic epidemiology.
    • Developing a 3-tiered framework for establishing and sustaining NGS and bioinformatics infrastructure.
    • Strategic planning informed by the experiences of the Global Emerging Infections Surveillance program.

    Main Results:

    • A comprehensive 3-tiered framework was developed for DoD genomic surveillance capabilities.
    • The strategy was established pre-pandemic, enabling a prepared response.
    • The network was positioned to utilize existing resources and scale with new funding.

    Conclusions:

    • A structured, expert-driven approach is effective for building genomic surveillance infrastructure.
    • Proactive development of genomic surveillance capabilities enhances pandemic preparedness.
    • The established framework supports ongoing monitoring of pathogens of military and public health importance.