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Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
Published on: June 9, 2014
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Dual Responsive Emulsions Based on Amphiphilic Elastin-like Polypeptide Bioconjugates.
Laurianne Simon1, Dongxu Zhou2, Anita Coeurvolan1
1ICGM, University Montpellier, CNRS, ENSCM, Montpellier 34293, France.
Bioconjugate Chemistry
|November 12, 2024
Summary
Smart elastin-like polypeptides (ELP-g-Bu) create stable emulsions for drug delivery. These smart polymers release loaded drugs in response to temperature or oxidation, showing potential for inflammatory disease treatments.
Area of Science:
- Biomaterials Science
- Polymer Chemistry
- Drug Delivery Systems
Background:
- Controlled drug release is crucial for therapeutic efficacy.
- Smart polymers offer tunable properties for advanced drug delivery.
- Natural polypeptides present opportunities for biocompatible delivery systems.
Purpose of the Study:
- To evaluate elastin-like polypeptides grafted with alkyl chains (ELP-g-Bu) as amphiphilic smart surfactants.
- To assess their ability to stabilize oil-in-water emulsions.
- To investigate stimuli-responsive release of loaded molecules.
Main Methods:
- Synthesis of ELP-g-Bu and characterization of physicochemical properties.
- Preparation and stability assessment of paraffin-oil-in-water emulsions.
- Drug loading with a lipophilic molecule and release studies under thermal and oxidative stimuli.
Main Results:
- ELP-g-Bu exhibited dual-responsive changes in critical aggregation concentration.
- Stable emulsions with a droplet size of 9 ± 1 μm were formed and maintained for a month.
- Complete drug release was triggered at 42 °C, with partial release under oxidation.
Conclusions:
- Amphiphilic ELP-g-Bu are effective macromolecular surfactants for creating stable emulsions.
- These smart polymers demonstrate dual-stimuli responsiveness for controlled drug release.
- The developed system shows promise for drug delivery applications, particularly in inflammatory diseases.
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