JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

Quantitative proteogenomic characterization in MUC1 and MUC4 in oral squamous cell carcinoma, oral potentially malignant disorders, and normal oral mucosa in carcinogenesis

  • 0Department of Oral Pathology, King George's Medical University, Lucknow, 226003, UP, India.
European Archives of Oto-Rhino-Laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (eufos) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery +

|

November 12, 2024

|

November 12, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Mucin 1 (MUC1) and Mucin 4 (MUC4) expression increases in oral premalignant disorders and oral squamous cell carcinoma. MUC1 indicates poorly differentiated tumors, while MUC4 suggests better prognosis in oral cancers.

Area Of Science

  • Biomedical Science
  • Oncology
  • Molecular Biology

Background

  • Mucins, like MUC1 and MUC4, are glycoproteins with vital biological roles.
  • Their altered expression is noted in various carcinomas, but research on oral malignancies is limited.
  • MUC1 and MUC4 are transmembrane mucins found in epithelial cells and body fluids.

Purpose Of The Study

  • To assess MUC1 and MUC4 gene expression patterns in oral squamous cell carcinoma (OSCC).
  • To evaluate MUC1 and MUC4 expression in oral premalignant disorders (OPMDs) including oral epithelial dysplasia (OED), oral submucous fibrosis (OSF), and oral lichen planus (OLP).
  • To compare MUC1 and MUC4 expression between OPMDs, OSCC, and normal oral mucosa (NOM).

Main Methods

  • Immunohistochemistry and quantitative reverse transcription PCR (qRTPCR) were used to analyze MUC1 and MUC4 expression.
  • The study included 63 cases of OSCC, OPMD, and NOM.
  • Statistical analysis was performed using one-way ANOVA and Chi-square tests.

Main Results

  • Overall immunoexpression of MUC1 and MUC4 significantly increased from normal oral mucosa to OPMDs and then to OSCC.
  • Staining intensity for MUC1 and MUC4 varied across subgrades of OSCC and OPMDs.
  • MUC4 expression was strongest in well-differentiated grades, whereas MUC1 expression was higher in poorly differentiated grades.

Conclusions

  • MUC1 and MUC4 are crucial biomarkers for monitoring OSCC and OPMD pathogenesis.
  • MUC1 expression correlates with highly undifferentiated grades, while MUC4 indicates more differentiated tumors.
  • These mucins can serve as valuable tumor markers for assessing severity and early detection of oral malignancies.

Keywords: