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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Related Experiment Video

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Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer
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High detail resolution cellulose structures through electroprinting.

Farnaz Rezaei1, Daniel O Carlsson2, Jimmy Hedin Dahlstrom2

  • 1Department of Materials Science and Engineering, Uppsala University, 75105, Uppsala, Sweden. Farnaz.rezaei@angstrom.uu.se.

Scientific Reports
|November 12, 2024
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Summary

Electroprinting precisely positions polymer fibers for 3D structures. This technique fabricates custom separation membranes with sub-micrometer features, though printing speed needs improvement.

Keywords:
Additive manufacturingCellulose acetateElectroprintingHigh-resolution 3D printing

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Area of Science:

  • Materials Science
  • Chemical Engineering
  • Biomaterials Engineering

Background:

  • Electrospinning fabricates micro/nanoscale polymer fibers but lacks precise fiber positioning.
  • Limited positioning accuracy in electrospinning hinders the creation of complex, designed structures.

Purpose of the Study:

  • To develop an electroprinting technique for enhanced control over fiber placement.
  • To investigate the fabrication of 3D structures with micrometer precision using electroprinting.
  • To explore the potential of electroprinting for creating customized separation membranes.

Main Methods:

  • Developed an electroprinting technique by reducing nozzle-collector distance for improved fiber positioning control.
  • Utilized cellulose acetate (CA) as a biomaterial for 3D membrane printing.
  • Evaluated parameters including CA concentration, molecular weight, printing speed, pattern, and applied voltage.

Main Results:

  • Achieved improved control over fiber positioning, enabling the fabrication of designed 3D structures at the micron scale.
  • Demonstrated the possibility of printing structures with inter-fiber distances as small as 3 µm.
  • Obtained fiber diameters at the sub-micrometer scale through optimized electroprinting parameters.

Conclusions:

  • Electroprinting offers a promising approach for fabricating customized separation membranes with high precision.
  • Optimized printing parameters allow for significant control over fiber diameter and spacing.
  • Printing speed remains a key challenge for further advancement of the electroprinting technique.