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Halogenated Agent Delivery in Porcine Model of Acute Respiratory Distress Syndrome via an Intensive Care Unit Type Device
Published on: September 24, 2020
Critically ill patients will benefit from single isolated laminar-air-flow wards by improving the environmental
Tianji Zhang1, Xiao Cui1, Xinlei He1
1Department of Intensive Care Unit, Peking University Third Hospital, North Garden Road, No. 49, Beijing, Haidian District, 100191, People's Republic of China.
Background:
Hospital-associated infection (HAI) is an important issue in intensive care units (ICUs). We still lack direct evidence on whether the ICU patients and/or the medical system can benefit from single isolated laminar-air-flow (LAF) wards.
Methods:
High-touched-surface (HTS) swabs from 5 sites in two kinds of wards with different ventilation systems were longitudinally collected for 16 S rRNA sequencing and Type IIB restriction site-associated DNA sequencing for Microbiome (2bRAD-M). Samples were collected for 3 months. The clinical data of patients admitted to different wards during the sampling time and the whole year were collected and compared.
Results:
The α-diversity of single wards with isolated LAF was significantly higher than open regions without LAF (p<0.01). β-diversity analysis showed differences between different wards and similarities among the same region. We also identified 3 genera attributed to the most difference between the two kinds of wards. 2bRAD-M analysis further revealed community divergence among different HTS sites. There was an overlap between HTS microbiome profiling and the clinically cultivated pathogens of patients with HAI. People in single wards had a better outcome than those in open regions (p<0.05), indicating that single wards had a protective effect for critically ill patients.
Conclusion:
Overall, there was a prominent difference in the microbiome community between single wards and open regions. Single wards had more balanced communities which may lead to better outcomes for patients. For critically ill patients, single ward is recommended when arranging and constructing.
Insights
Single laminar-air-flow (LAF) wards significantly improve the microbiome diversity and patient outcomes in intensive care units (ICUs). These wards offer a protective effect for critically ill patients compared to open regions.
Area of Science:
- Microbiology
- Environmental Science
- Healthcare Management
Background:
- Hospital-associated infections (HAIs) pose a significant challenge in intensive care units (ICUs).
- Evidence regarding the benefits of single isolated laminar-air-flow (LAF) wards for ICU patients and healthcare systems remains limited.
Purpose of the Study:
- To investigate the impact of single isolated LAF wards on the microbiome composition of high-touched surfaces (HTS).
- To evaluate the clinical outcomes of patients in different ward types.
Main Methods:
- Longitudinal collection of HTS swabs from two ward types with distinct ventilation systems.
- 16S rRNA sequencing and 2bRAD-M for microbiome analysis.
- Comparison of clinical data from patients admitted to different wards.
Main Results:
- Single LAF wards exhibited significantly higher alpha-diversity compared to open regions (p<0.01).
- Distinct microbiome communities were observed between ward types, with similarities within regions.
- An overlap was found between HTS microbiome profiles and clinically relevant pathogens.
- Patients in single wards demonstrated better outcomes (p<0.05), indicating a protective effect.
Conclusions:
- Single wards foster more balanced microbiome communities, potentially improving patient outcomes.
- The study recommends single wards for critically ill patients in healthcare facility design and construction.
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